保心丸对多柔比星致扩张型心肌病家兔肝功能的影响

Study of Baoxin Pill on Liver Function in Doxorubicin-induced Dilated Cardiomyopathy Rabbits

目的:探讨保心丸对扩张型心肌病家兔肝功能的影响。方法:新西兰兔72只,除正常组6只外,其余家兔采用耳缘iv 0.75 mg·kg-1多柔比星方法复制家兔扩心病模型,每周2次,于第4周B超检查记录家兔心率(HR),主动脉内径(AO),左室舒张期末内径(LVEDD),左室收缩期末内径(LVESD),计算左室射血分数(LVEF)及每分钟搏出量(SV),将造模成功的家兔随机分为模型组、保心丸高、中、低剂量组(2.7,1.35,0.675 g·kg-1)及卡托普利组2.5 mg·kg-1,连续ig给药30 d,每天1次,并于第8周取血清检测丙氨酸氨基转移酶(ALT),天门冬氨基转移酶(AST),碱性磷酸酶(ALP),γ-谷氨酰转移酶(γ-GT),总蛋白(TP),白蛋白(ALB),肌酸激酶(CK)的含量;心室取材,HE染色观察心脏组织的病理变化。结果:与正常组比较,第4周B超检测模型组射血分数及每分钟搏出量明显降低(P<0.05,P<0.01),表明扩张型心肌病模型成功。第8周时血清肝功检测,与正常组比较,模型组ALT,AST,γ-GT,CK含量明显升高(P<0.05,P<0.01),TP含量明显降低(P<0.05);与模型组比较,保心丸各剂量组及卡托普利组明显降低ALT,AST,γ-GT,CK含量(P<0.05,P<0.01),明显升高TP含量(P<0.05)。病理观察显示,模型组部分心肌萎缩,部分心肌细胞轻-中度浊肿,或轻度脂肪变性,间质纤维组织增生,血管充血,各给药组上述病理变化均有明显减轻。结论:保心丸对多柔比星致家兔扩张型心肌病肝功能有一定的保护作用。

Objective： To study the effect of Baoxin pill on liver function in the rabbit with dilated cardiomyopathy（DCM）. Method： Seventy-two rabbits were randomly divided into the normal group,the model group,the high-,medium-,low-dose Baoxin pill groups（2. 7,1. 35,0. 675 g·kg- 1）,and the captopril group（2. 5 mg·kg- 1）. The DCM model was induced in rabbits by injecting doxorubicin（0. 75 mg·kg- 1） via the ear vein twice weekly for 4 weeks. The heart rate,aortic dimension,left ventricular and diastolic diameter,left ventricular and systolic diameter were measured,and the left ventricular ejection fraction（LVEF） and the stroke volume（SV） were calculated at the fourth week. After continuous intragastric administration of the corresponding medicines for 30 days,the levels of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,alkaline phosphatase（ALP）,γ-glutamyltransferase（γ-GT）,total protein（TP）,albumin（ALB）,creatine kinase（CK）were measured at the eighth week. The pathological changes of heart tissue were observed by HE staining. Result：Compared with normal group,the LVEF and SV decreased at the fourth week,the levels of ALT,AST,γ-GT,ALB,TP,CK increased,the level of TP decreased at eighth weeks in the model group（P〈0. 05,P〈0. 01）.Compared with model group,the levels of ALT,AST,γ-GT,ALB,TP,CK decreased,the level of TP increased at eighth weeks in the Baoxin pill groups（P〈0. 05,P〈0. 01）. Moreover,the alleviated pathological changes in heart tissues were observed in the Baoxin pill groups. Conclusion： Baoxin pill has a certain protective effect on liver function in rabbits with dilated cardiomyopathy induced by adriamycin.