前列腺增生合并前列腺炎的临床分析及MMP-9的表达与意义

Expression of matrix metalloproteinase 9 in benign prostatic hyperplasia with chronic prostatitis and its clinical significance

目的通过检测良性前列腺增生(BPH)合并前列腺炎(CP)患者基质金属蛋白酶(MMP-9)蛋白的表达,结合临床特点,探讨MMP-9在BPH中的作用与意义。方法选择BPH患者68例,根据是否合并CP将其分为,单纯组(21例)和合并组(47例)。观察并记录两组患者年龄、前列腺体积、国际前列腺症状评分(IPSS)和生活质量评分(QOL);ELISA检测患者血清中前列腺特异抗原(PSA)浓度;ELISA和Western blot法检测前列腺组织中MMP-9蛋白的表达水平。结果单纯组患者年龄、前列腺体积、IPSS和QOL均明显低于合并组(P<0.05)。ELISA结果显示血清中PSA与组织中MMP-9浓度成正相关,合并组高于单纯组为(P<0.05)。Western blot结果表明合并组中MMP-9蛋白表达水平较高(P<0.05)。结论 BPH合并CP患者MMP-9蛋白表达水平与各项临床指标呈正相关,有利于BPH患者和BPH合并CP患者的鉴别诊断。

[Objective]To detect matrix metalloproteinase 9（MMP-9） protein expression in patients of benign prostatic hyperplasia（BPH） combined with chronic prostatitis（CP）, combined with the clinical characteristics, to reveal its role and significance in BPH.[Methods]The patients with BPH（68 cases） were divided into simple group（21 cases） and combined group（47 cases）. The age, prostate volume, International Prostate Symptom Score（IPSS）and quality of life score（QOL） were observed and recorded in both groups. The concentration of prostate-specific antigen（PSA） in serum was detected by enzyme-linked immunosorbent assay（ELISA）. The expression levels of MMP-9 protein were detected by ELISA and Western blot. [Results]The age, prostate volume, IPSS and QOL in the simple group were significantly lower than those in the combined group（P〈0.05）. ELISA results showed that PSA concentration was positively correlated to MMP-9 level, their concentrations were higer in the combined group than in the simple group（P〈0.05）. Western blot results showed that MMP-9 protein expression level was higher in the combined group（P〈0.05）. [Conclusions]In patients of BPH combined with CP MMP-9 protein expression level is positively correlated with clinical indices, which is conducive to differential diagnosis between BPH alone and BPH combined with CP.