摘要
We reported in this manuscript that TGF-β1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly inhibited the TGF-β1-induced apoptosis and PAI-1 promoter activity. Treatment of cells with TGF-β1 activates p38. Furthermore, over-expression of dominant negative mutant p38 also reduced the TGF-β1-induced apoptosis. The data indicate that the activation of p38 is involved in TGF-β1-mediated gene expression and apoptosis.
We reported in this manuscript that TGF-beta1 induces apoptosis in AML12 murine hepatocytes, which is associated with the activation of p38 MAPK signaling pathway. SB202190, a specific inhibitor of p38 MAPK, strongly inhibited the TGF-beta1-induced apoptosis and PAI-1 promoter activity. Treatment of cells with TGF-beta1 activates p38. Furthermore, over-expression of dominant negative mutant p38 also reduced the TGF-beta1-induced apoptosis. The data indicate that the activation of p38 is involved in TGF-beta1-mediated gene expression and apoptosis.
基金
grants fromthe Chinese Academy of Sciences (No. KJ951-BI608), the National Natural Sciences FOundation ofChina (No. 39625007 and
关键词
转化生长因子Β
细胞凋亡
P38
肝细胞
信号传导
Animals
Apoptosis
Cells, Cultured
DNA Fragmentation
Enzyme Inhibitors
Gene Expression Regulation, Enzymologic
Genes, Reporter
Genetic Vectors
Hepatocytes
Imidazoles
MAP Kinase Signaling System
Mice
Mitogen-Activated Protein Kinases
Mutation
Phosphorylation
Plasminogen Activator Inhibitor 1
Pyridines
Research Support, Non-U.S. Gov't
Transfection
Transforming Growth Factor beta
p38 Mitogen-Activated Protein Kinases
