洛铂对体外培养人卵巢癌细胞增殖的抑制作用

Inhibition of lobaplatin on cultured human ovarian cancer cell proliferation in vitro

目的观察洛铂（LBP）对体外培养人卵巢癌细胞增殖的抑制作用。方法选用7种人卵巢癌细胞株SK-OV．3、ES-2、OVCAR-8、3AO、SW626、A2780、OVCAR-3，以顺铂（DDP）、卡铂（CBP）为对照，评价LBP单药或联合紫杉醇（vrx）、多西他赛（TXT）对体外培养人卵巢癌细胞增殖的抑制作用及其联合用药效应。结果LBP、DDP、CBP单药均可明显抑制体外培养人卵巢癌细胞增殖，IC5。分别为1．5-13．8、0．9-8．1和14．6-145．7μmol·L-1 LBP、DDP、CBP联合P1、X、TXT的效应具有细胞特异性，其中，LBP联合TXT在SK-OV-3、OVCAR-8细胞中显示相加效应。结论LBP单药具有明确的体外抗人卵巢癌细胞增殖活性．其活性与DDP相当，强于CBP；LBP联合TXT对卵巢癌细胞有可能产生相加的抗肿瘤作用，但有细胞特异性。

AIM To observe the effects of lobaplatin （LBP） treatment on ovarian cancer cells in vitro. METHODS Seven kinds of human ovarian cancer cell lines SK-OV-3, ES-2, OVCAR-8, 3AO, SW626, A2780, and OVCAR-3 were used to observe the anti-tumor effects of cisplatin （DDP）, carboplatin （CBP）, and LBP, alone or in combination with paclitaxel （PTX）, docetaxel （TXT）, on inhibition of human ovarian cancer cell proliferation in vitro. RESULTS LBP, DDP, CBP could significantly inhibit human ovarian carcinoma cells＇ growth in vitro. Icso of LBP was 1.5 - 13.8 μmol·L-1, DDP was 0.9 - 8.1 μmol·L-1, CBP was 14.6 - 145.7 μmol·L-1. When combining LBP, DDP, or CBP with PTX and TXT, the anti-tumor effect was cell- specific. LBP in combination with TXT showed additive effects in SK- OV- 3, OVCAR- 8 cells. CONCLUSION LBP has an obvious anti-human ovarian cancer cell proliferation activity, which is similar to DDP and better than CBP. LBP plus TXT has additive anti-tumor effects in certain ovarian cancer cells.