摘要
目的探讨CD200减轻帕金森病(PD)病变区域炎症反应的作用。方法制备1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)小鼠PD模型,取脑组织。检测CD200及其受体CD200R的表达和ATP敏感性钾通道(KATP通道)不同亚基在小胶质细胞(MG)的表达;检测MG的细胞因子;分析外源性重组CD200对KATP通道释放的影响。结果 PD模型小鼠CD200及CD200R的表达下调,MG的IFN-γ、TNF-α和IL-1β的释放增多。外源性重组CD200能够抑制MG中ATP的释放,MG中KATP通道的开放增加,1-甲基-4-苯基-吡啶离子(Mpp+)引起的MG激活及炎症因子和氧化因子的释放受到抑制。结论CD200通过抑制MG内ATP的水平,促进MG中KATP通道的开放,抑制MG的活化及相关炎症因子的释放,减轻黑质纹状体区域的炎症反应,从而起到了保护多巴胺神经元的作用。
Objective To explore the role of CD200 in attenuating the inflammatory response in the substantia nigra area of mouse model with Parkinson′s disease（PD） .Methods PD model of mice was established by MPTP injection and brain tissues were taken for the examinations .The expressions of CD200 and its receptor （CD200R） were detected .The expressions of ATP-sensitive potassium channel and cytokines in the subunits were determined .The effect of exogenous recombinant CD200 on the opening of ATP-sensitive potassium channel in vitro was analyzed .Results The expressions of CD200 ,CD200R ,and the release of IFN-γ,TNF-αand IL-1βin the subunits were all decreased in PD mice .The exogenous recombinant CD200 inhibited the ATP release and promoted the opening of ATP-sensitive potassium channel in microglia .Mpp ＋-induced microglia activation and release of inflammatory factors and oxygen free radicals were depressed .Conclusion CD200 might protect the dopaminergic neuron by attenuating neuroinflammation in the substantia nigra area via reducing ATP release ,promoting the opening of ATP-sensitive potassium channel ,inhibiting microglia activation and release of inflammatory factors and oxygen free radicals .
出处
《江苏医药》
CAS
2015年第8期876-879,共4页
Jiangsu Medical Journal
