协同刺激分子B7-H1在自发性流产患者的表达及其生物学意义

Expression and biological significance of costimulatory molecule B7-H1 in patients with spontaneous abortion

目的研究协同刺激分子B7-H1在自发性流产患者的表达及其生物学意义。方法 ELISA法检测正常未孕者(A组,52例)、正常妊娠者(B组,49例)、自发性流产患者(C组,52例)和自发性流产后再妊娠者(D组,17例)血清可溶性B7-H1(sB7-H1)的含量。免疫组化法检测正常妊娠行人工流产术患者(E组,20例)和自发性流产患者(F组,21例)绒毛膜和蜕膜中B7-H1的表达。结果 A、B、C、D组血清sB7-H1含量无统计学差异(P>0.05)。E组蜕膜中B7-H1的表达高于F组(P<0.05),但两组绒毛膜中B7-H1的表达无统计学差异(P>0.05)。结论 B7-H1参与了正常妊娠过程;其异常表达对自发性流产的发生有重要意义。

Objective To study the expression and biological significance of costimulatory molecule B7-H1 in the patients with spontaneous abortion .Methods Serum levels of soluble B7-H1 （sB7-H1） in nonfertile women （group A ,52 cases） ,normal pregnancy women （group B ,49 cases） , patients with spontaneous abortion （group C ,52 cases ） and pregnancy women with a history of spontaneous abortion（group D ,17 cases） were determined by ELISA .The expressions of B7-H1 in chorion and decidua tissues of normal pregnancy women underwent artificial abortion （group E , 20 cases） and of patients with spontaneous abortion （group F ,21 cases ） were detected by immunohistochemistry .Results There was no significant difference in serum content of sB7-H1 among groups of A ,B ,C and D（P〉0 .05） .The expression of B7-H1 in decidua tissues was higher in group E than that in group F （P〈0 .05） ,which in chorion tissues showed no significant difference between two groups（P〉0 .05） .Conclusion B7-H1 participates in the process of normal pregnancy and abnormal expression of B7-H1 has an important significance in the occurrence of spontaneous abortion .