五鹤续断总皂苷抗皮肤衰老的作用及其机制

Study of anti-aging effect and its mechanism of total saponins of Wu-He Dipsacus asper on skin of mouse-aging model

目的:研究五鹤续断总皂苷抗皮肤衰老的作用及其机制。方法:将48只小鼠随机分为空白对照组、模型组、五鹤续断低、中、高剂量组和阳性对照组(n=8)。采用5%D-半乳糖(0.025 ml/(g·d))颈背部皮下注射制备皮肤衰老小鼠模型,五鹤续断低、中、高剂量组灌服五鹤续断总皂苷水溶液(50 ml/(kg·d)、100 ml/(kg·d)、200ml/(kg·d)),阳性对照组灌服维生素E(50 mg/(kg·d)),连续给药42 d后,检测各组小鼠皮肤组织羟脯氨酸(HYP)和脂褐质(LF)的含量,血清和皮肤组织过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的活性与丙二醛(MDA)的含量。结果:与空白对照组比,模型组小鼠皮肤组织HYP含量明显减少,LF含量明显增多,血清和皮肤组织CAT、GSH-Px、SOD活性显著降低,MDA含量明显增加;与模型组比,五鹤续断低、中、高剂量和阳性对照组皮肤组织HYP含量明显增多,LF含量明显减少,血清和皮肤组织CAT、GSH-Px、SOD活性显著增强,MDA含量明显减少;与低剂量组比,高剂量和阳性对照组皮肤组织HYP含量明显增多,LF含量明显减少,血清和皮肤组织CAT、GSH-Px、SOD活性显著增强,MDA含量明显减少;血清和皮肤组织SOD活性与皮肤组织HYP呈显著正相关,与LF呈显著负相关。结论:五鹤续断总皂苷具有明显的抗皮肤衰老作用,其作用机制与抗氧化损伤密切相关。

Objeelive： To Study the effect of anti-aging and its mechanism of total saponins of Wu-He Dipsacus asper on skin of mice-ag- ing model. Methods： Forty-eight mice were randomly divided into blank control group, model group, low-Dipsaeus group, medium-Dipsacus group, high-Dipsacus group and positive control group（ n = 8）. The mouse model of skin aging was established by nape subcutaneous injection of 5 % D-galactose （0. 025 ml/（ g· d））, the mouse of low-Dipsacus group , medium-Dipsacus group, high-Dipsacus group were administered with total saponins of Wu-He Dipsacus asper（ 50 ml/（ kg·d）, 100 ml/（ kg· d）, 200 ml/（kg·d） ）, the mice of the positive control group were administered with vitamin E（50 mg/（kg·d） ）for 42 d. The content of hydroxyproline （HYP） and lipofuscin （LF） were measured in skin of each group mice, the activity of catalase （CAT）,glntathione peroxidase （GSH-Px） ,superoxide dismutase （SOD） and the content of malondi- aldehyde （MDA） were determined in serum and skin of each group mice. Results： Compared with blank control group, the content of HYP decreased significantly and the content of LF increased significantly in skin, the activities of CAT, GSH-Px and SOD decreased significantly and the content of MDA increased significantly in serum and skin of model group; Compared with model group, the content of HYP increased significantly and the content of LF decreased significantly in skin, the activities of CAT, GSH-Px and SOD enhanced significantly and the con- tent of MDA decreased significantly in serum and skin of low-I）ipsacus group, medium-Dipsacus group, high-Dipsacus group and positive control group; Compared with low-Dipsacus group, the content of HYP increased significantly and the content of LF decreased significantly in skin, the activities of CAT, GSH-Px and SOD enhanced significantly and the content of MDA decreased significantly in serum and skin of high-Dipsacus group and positive control group; The activity of SOD in sertan and skin had a significant positive correlation with the content of HYP, and a significant negative correlation with LF in skin. Conclusion： Total saponins of Wu-He Dipsacus asper have obvious effect of anti-agng on skin of mouse-aging model , its mechanism is closely related to oxidative damage.