摘要
目的观察右美沙芬对糖尿病大鼠视网膜神经病变中Caspase-3表达的影响。方法健康成年Sprague Dawley大鼠48只,12只作为正常对照组(D组),36只大鼠链脲佐菌素腹腔注射建立糖尿病大鼠模型,并随机分为糖尿病模型组(A组),10 mg·kg-1右美沙芬治疗组(B组;造模成功后一次性腹腔注射10 mg·kg-1右美沙芬),30 mg·kg-1右美沙芬治疗组(C组;造模成功后一次性腹腔注射30 mg·kg-1右美沙芬),每组各12只。各组分别于4周末、8周末各处死大鼠6只,行免疫组织化学法检测视网膜组织Caspase-3蛋白的表达。结果视网膜Caspase-3光密度值:A组4周、8周时分别为0.256±0.031和0.374±0.234,B组分别为0.244±0.122和0.352±0.152,C组分别为0.174±0.021和0.256±0.138,D组分别为0.074±0.018和0.072±0.233。A组4周Caspase-3蛋白表达主要见于神经节细胞层,8周时阳性表达进一步增加,扩展到内核层。C组Caspase-3表达变化规律与A组基本一致,但4周、8周时阳性表达量均低于A组(均为P<0.05),A组与B组间4周和8周时Caspase-3阳性表达量差异均无统计学意义(均为P>0.05)。D组4周、8周时大鼠视网膜上几乎无Caspase-3阳性表达。结论早期糖尿病大鼠视网膜Caspase-3参与了糖尿病视网膜病变的发病机制,右美沙芬可以抑制Caspase-3蛋白的表达,对糖尿病视网膜病变有一定的治疗作用。
Objective To observe the effect of dextromethorphan on the expres- sion of Caspase-3 in retinal nerve cell of rats with diabetic retinopathy. Methods For- ty-eight adult Sprague Dawley rats were randomly divided into normal control group（ 12 cases,group D） and diabetic model group （36 cases were intraperitoneally injected with streptozotocin）. Thirty-six rats were successfully made into diabetic models and ran- domly divided into diabetes meUitus group （group A）, 10 mg · kg-1 dextromethorphan group （group B） and 30 mg · kg-1 Dextromethorphan group （group C）, 12 cases in each group. The six rats of every group were sacrificed at 4 weeks and 8 weeks, the ex- pression of Caspase-3 in retina was studied by immunohistochemistry. Results Aver- age optical density value of Caspase-3 in retina of A group at 4 weeks and 8 weeks were 0.256 ± 0.031 and 0.374 ± 0.234, respectively, which in group B were 0. 244 ± 0. 122 and 0.352 ±0. 152 ,respectively ,group C were 0. 174 ±0.021 and 0.256±0. 138 ,respectively, group D were 0.074 ±0.018 and 0.012±0.233 ,respectively. At 4 weeks of group A,the positive expression of Caspase-3 was mainly located in ganglion cell layer, and the posi- tive expression was increased and extended to inner nuclear layer at 8 weeks. The trend of Caspase-3 expression was consistent between group C and group A, but the positive expression at 4 weeks and 8 weeks in group C were less than those in group A （ all P 〈 0.05 ）. There was no significant difference in Caspase-3 positive expression at 4 weeks and 8 weeks between group A and B （ all P 〉 0.05 ）. Positive expression of Caspase-3 was almostly not found in retina of group D at 4 weeks and 8 weeks. Conelus｜on In early diabetic ra^s, Caspase-3 plays a role in the development of diabetic retinopathy. Dextromethorphan can inhibit the expression of Caspase-3 ,which has certain therapeu- tic effect on diabetic retinopathy.
出处
《眼科新进展》
CAS
北大核心
2015年第5期432-434,共3页
Recent Advances in Ophthalmology
