乙肝后肝硬化患者淋巴细胞亚群的变化

Changes of lymphocyte subsets in liver cirrhosis patients after hepatitis B

目的观察慢性乙型肝炎及乙肝后肝硬化患者免疫功能的改变,探讨其免疫功能改变的原因。方法用流式细胞仪对34例慢性乙肝及39例乙肝后肝硬化患者分别测定静脉血淋巴细胞亚群(CD3+、CD4+、CD8+、CD56+、CD19+)的数值,并与健康体检者的淋巴细胞亚群数值比较。用实时荧光定量PCR测定乙肝后肝硬化HBV-DNA的含量。结果健康对照组、慢性乙型肝炎组及乙肝后肝硬化组CD3+、CD4+、CD8+、CD56+、CD19+两两比较,差异均有统计学意义(P<0.05);乙肝后肝硬化Child A组、Child B、Child C组CD3+、CD4+、CD8+、CD56+、CD19+两两比较,差异均有统计学意义(P<0.05),HBV-DNA含量相比,差异无统计学意义(P>0.05)。结论随着乙肝患者病情恶化,机体免疫系统的紊乱逐渐加重。

Objective To observe the changes of lymphocyte subsets of patients with chronic virus hepatitis B and liver cirrhosis after hepatitis B , and investigate the reasons for the changes of lymphocyte subsets .Methods Lympho-cyte subsets （ CD3^＋, CD4^＋, CD8^＋, CD56^＋, CD19^＋） of peripheral blood in 34 patients with chronic virus hepatitis B and 39 patients with liver cirrhosis after hepatitis B were measured by flow cytometer .Then compared with healthy physi-cal examination people .Real-time fluorescent quantitative PCR was used on patients with liver cirrhosis after hepatitis B detected HBV-DNA contents in serum .Results The CD3 ＋, CD4 ＋, CD8 ＋, CD56 ＋, CD19 ＋in healthy control group , chronic hepatitis B group and liver cirrhosis after hepatitis B group compared with each other were significant difference （ P〈0 .05 ） .The CD3^＋, CD4^＋, CD8^＋, CD56^＋, CD19^＋ in liver cirrhosis after hepatitis B Child A group , Child B group and Child C group compared with each other were significant difference （ P〈0 .05 ） , While HBV-DNA contents was not significant difference （P〉0.05）.Conclusion The immune function of patients with chronic hepatitis B disor-der gradually as the deterioration of diseases .