肝硬化肠道细菌移位大鼠多器官TNF-α的变化

Changes of TNF-α levels in multiple organs of liver cirrhosis rats with intestinal bacterial translocation

目的探索肝硬化肠道细菌移位(BT)大鼠多脏器TNF-α的变化及其与组织损伤的相关性。方法实验大鼠分为正常对照组(NC组)、BT阴性组(BT-组)及BT阳性组(BT+组);ELISA法检测各组大鼠回肠、肝、肾、脑、外周血中TNF-α含量;HE染色评估组织损伤;探讨组织损伤评分与其相应组织及外周血TNF-α含量的相关性。结果在回肠、肝、脑、外周血中,BT+组的TNF-α均较BT-组显著升高(P<0.05);其增高水平与相应组织的损伤程度呈显著正相关。结论肝硬化BT所诱发的TNF-α水平升高在回肠、肝、脑组织的损伤中发挥重要作用,从而为临床肝硬化发病机制探讨及防治BT相关并发症提供帮助。

Objective To study the changes of tumor necrosis factor-α（ TNF-α） in multiple organs and its relation-ship with the organ injury in liver cirrhosis rats with intestinal bacterial translocation （ BT） .Methods Rats were divid-ed into 3 groups, normal control （NC） group, BT negative （BT-） group and BT positive （BT ＋） group.ELISA was used to measure the TNF-αlevels in ileum, liver, kidney and brain .Pathological analysis was used to measure the organ injury by Hematoxylin-eosin staining .The relationship of TNF-αwith the organ injury was studied by statistical analysis . Results TNF-αlevels in livers, ileums, brains, peripheral blood specimens in the BT ＋group were significantly higher than those in BT -group （P〈0.05）.Pathological injury of ileums, livers, and brains were positively related to the TNF-αlevel in corresponding tissues and blood （r〉0, P〈0.05）.Conclusion BT-induced TNF-αinvolves in the multiple organ injury after liver cirrhosis , therefore benefiting the prevention and treatment of BT related complications after liver cirrhosis .