缺氧预处理骨髓间充质干细胞对大鼠脊髓缺血再灌注损伤保护作用的研究

The protective effect of hypoxic-preconditioned BMSCs on spinal cord tissue after ischemia reperfusion injury in rats

目的 探讨缺氧预处理骨髓间充质干细胞对大鼠脊髓缺血再灌注损伤的影响.方法 SD大鼠18只,按随机数表法分为3组（n=6）,假手术组（Sham组）仅行单纯手术操作不进行脊髓缺血再灌注;对照组（Control组）行脊髓缺血再灌注处理;缺氧预处理细胞组（HP-BMSCs）在脊髓缺血再灌注损伤前2d鞘内注射经过缺氧预处理的BMSCs.对照组、HP-MSCs组行脊髓缺血10 min,观察48 h.伊文思蓝染色观察血脊髓屏障的通透性变化,HE染色检测脊髓组织的病理学变化.结果 与对照组相比,HP-BMSCs组脊髓损伤组织伊文思蓝浓度显著降低（P＜0.05）,病理学损伤显著减轻.结论 缺氧预处理的骨髓间充质干细胞移植可通过减轻大鼠血脊髓屏障完整性的破坏起到对脊髓缺血再灌注损伤的保护作用.

Objective To investigate the protective effect of hypoxic-preconditioned bone marrow mesenchymal stem cells （BMSCs） on spinal cord tissue after ischemia reperfusion injury.Methods Healthy adult Sprague Dawley （SD） rats weighing 200 ～ 250 grams （g） were randomly divided into 3 groups with 6 animals in each group：The sham group received simple surgical manipulation without ischemia/reperfusion treatment;The spinal cord ischemia/reperfusion group （Control group） only received spinal cord ischemia/reperfusion surgery.The hypoxic preconditioned BMSC transplantation group （HP-MSCs group） was injected with hypoxic preconditioned BMSCs 2 days before ischemia/reperfusion.The control group,HP-MSCs group received spinal cord ischemia/reperfusion for 10 min and observed for 48 h.The permeability of the blood-spinal cord barrier was examined with Evans blue （EB）,and the histomorphology changes were observed with hematoxylin and eosin （HE） staining.Results EB red fluorescence was significantly weakened in the HP-MSCs group than that in the Control group （P 〈 0.05）,and more intact motor neurons were found in the lumbar spinal cords in the HP-MSCs group than that in the Control group （P 〈0.05）.Conclusions The hypoxic-preconditioned BMSCs could effectively attenuate spinal cord ischemia reperfusion injury,it may be associated with protective effect of the blood-spinal cord barrier integrity.