骨形成蛋白3在肝门部胆管癌组织中的表达及其临床意义

Expression and clinical significance of bone morphogenetic protein 3 in hilar cholangiocarcinoma tissues

目的:探讨肝门部胆管癌组织中骨形成蛋白3(BMP3)的表达情况及其临床意义。方法:收集30例肝门部胆管癌的临床标本,应用real-time PCR和Western blot检测BMP3 mRNA和蛋白在癌组织和癌旁组织的相对表达情况。收集103例肝门部胆管癌石蜡标本,免疫组织化学法检测肿瘤组织中BMP3蛋白的表达,并分析其与临床病理的关系。结果:全组30例肝门部胆管癌组织中,22例癌组织BMP3蛋白和mRNA的表达水平较癌旁组织明显下降。免疫组织化学方法显示103例肝门部胆管癌组织中,87例阴性,16例弱阳性,BMP3蛋白的表达与肿瘤TNM分期、淋巴结转移、肿瘤的分化程度密切相关(P<0.05)。结论:肝门部胆管癌组织中BMP3基因处于抑制状态,其表达的下调可能与肝门部胆管癌的发生、发展密切相关。

AIM： To investigate the expression and clinical significance of bone morphogenetic protein 3 （BMP3） in hilar cholangiocarcinoma tissues.METHODS： Thirty cases of hilar cholangiocarcinoma specimens were col-lected.The expression of BMP3 at mRNA and protein levels in the tumor tissues and paracancerous tissues was detected by real-time PCR and Western blot.The hilar cholangiocarcinoma paraffin-embedded specimens （n=103） were collected. The protein expression of BMP3 was determined by immunohistochemical method, and the relationship of BMP3 protein ex-pression with clinical pathological characteristics was evaluated.RESULTS：In the 30 patients with hilar cholangiocarcino-ma, the expressions of BMP3 protein and mRNA in 22 cases of tumor tissues were significantly decreased compared with the adjacent normal tissues.The results of immunohistochemistry showed that 87 cases were negative and 16 cases were weakly positive in all 103 cases of hilar cholangiocarcinoma.The expression of BMP3 protein was associated with the tumor TNM staging, lymph node metastasis and tumor differentiation （P〈0.05）.CONCLUSION：BMP3 gene might be inhibited in human hilar cholangiocarcinoma.The down-regulation of BMP3 gene might be associated with the carcinogenesis and devel-opment of hilar cholangiocarcinoma.