DEC1基因过表达对人食管癌ECA109细胞增殖及侵袭能力的影响

Effect of DEC1 gene over-expression on proliferation and invasion abilities of human esophageal cancer ECA109 cells

目的:探讨DEC1基因过表达对人食管癌ECA109细胞增殖和侵袭能力的影响及可能机制。方法:将质粒pc DNA3.1(-)/DEC1(DEC1组)和pc DNA3.1(-)(vector组)利用脂质体分别转染至人食管癌ECA109细胞中,通过real-time PCR检测转染48 h后的细胞内DEC1 mRNA表达,Western blot分别检测转染72 h后细胞内DEC1、基质金属蛋白酶9(MMP9)及细胞周期蛋白cyclin D1的蛋白表达;采用CCK-8实验、平板集落实验及Transwell实验分别检测DEC1过表达对细胞的增殖和侵袭能力的影响。结果:与vector组相比,DEC1组中DEC1的表达明显增高(P<0.01);cyclin D1和MMP9的表达明显降低(P<0.05);细胞增殖与侵袭能力明显受到抑制(P<0.01)。结论:过表达DEC1可明显抑制ECA109细胞的增殖和侵袭能力,DEC1可能通过影响MMP9和cyclin D1参与其中。

AIM：To investigate the effect of DEC1 gene over-expression on the proliferation and invasion abili-ties of human esophageal cancer ECA109 cells.METHODS： ECA109 cells were transfected with plasmid pcDNA3.1 （-）/DEC1 （DEC1 group） or pcDNA3.1 （-） （vector group）.The mRNA and protein levels of DEC1, cyclin D1 and MMP-9 were evaluated by real-time PCR and Western blot, respectively.The effects of DEC1 over-expression on the prolif-eration and invasion abilities of the ECA109 cells were evaluated by CCK-8 assay, colony formation assay and Transwell test respectively.RESULTS：The DEC1 expression level in ECA109 cells in DEC1 group was significantly higher than that in vector group （P〈0.01）, but the levels of MMP9 and cyclin D1 expression were opposite （P〈0.01）.However, both the proliferation and invasion abilities of ECA109 cells in DEC1 groups decreased significantly as compared with those in vector group （P〈0.05）.CONCLUSION：The over-expression of DEC1 significantly inhibits the proliferation and invasion of ECA109 cells, which may be involved in the expression of cyclin D1 and MMP9.