miR-26a-5p调控MCL-1在孕产妇子痫前期中表达的临床意义

Clinical significance of miR-26a-5p-regulated MCL-1 expression in preeclampsia

目的:探讨microRNA-26a-5p(miR-26a-5p)调控髓细胞白血病因子1(myeloid cell leukemia-1,MCL-1)表达在孕产妇子痫前期发生发展中的临床意义。方法:收集正常妊娠21例、妊娠期高血压孕产妇13例、轻度子痫前期15例和重度子痫前期26例共4组孕产妇血浆及胎盘组织,用real-time PCR分析其血浆及胎盘组织中miR-26a-5p及胎盘组织中MCL-1 mRNA的表达,Western blotting分析胎盘组织中MCL-1蛋白的表达,并分析其表达的临床意义。结果:随着病情进展,miR-26a-5p在孕产妇血浆和胎盘中表达逐步增高(P<0.01),而其胎盘组织中MCL-1 mRNA的表达明显降低(P<0.01),二者呈明显负相关(P<0.01);胎盘组织中miR-26a-5p与MCL-1蛋白表达呈明显负相关(P<0.01)。孕产妇血浆及胎盘组织中miR-26a-5p表达上调与孕龄、孕妇血浆白蛋白水平及胎儿体重呈明显负相关,而与孕产妇血压和尿蛋白水平呈明显正相关(P<0.01),胎盘组织中MCL-1表达下调与此相反。结论:miR-26a-5p高表达通过下调MCL-1的表达参与子痫前期的发生与发展。

AIM：To investigate the clinical significance of microRNA-26a-5p （miR-26a-5p）-regulated mye-loid cell leukemia-1 （MCL-1） expression in the development of maternal preeclampsia.METHODS：Plasma and placen-tal tissues were collected from 21 cases of normal pregnancy, 13 cases of maternal gestational hypertension, 15 cases of mild preeclampsia and 26 cases of severe preeclampsia.The levels of plasma and placental miR-26a-5p and placental MCL-1 mRNA were detected by real-time PCR.Western blotting analysis was used to determine the protein expression of placen-tal MCL-1.The clinical significance of the above parameters was also analyzed.RESULTS：miR-26a-5p expression gradu-ally increased（P〈0.01） in the 4 groups of maternal plasma and placentas with the disease development, and the mRNA expression of MCL-1 was significantly reduced in the placentas （P〈0.01）, both showing a significant negative correlation （P〈0.01）.Meanwhile, the expression of miR-26a-5p and MCL-1 protein in the placental tissues was negatively correla-ted （P〈0.01）.The miR-26a-5p up-regulation in maternal plasma and placental tissues was negatively correlated with ges-tational age, maternal plasma albumin levels and fetal weight, while it was positively correlated with maternal blood pres-sure and urinary protein level （P〈0.01）, which was in contrary to the down-regulation of placental MCL-1.CONCLU-SION：Up-regulation of miR-26a-5p is involved in the occurrence and development of preeclampsia by down-regulation of MCL-1.