摘要
目的:建立参芎葡萄糖注射液中总糖含量的良好测定方法。方法:采用紫外分光光度法和蒽酮-硫取法,以葡萄糖为对照品,对参芎葡萄糖注射液中总糖进行含量测定。结果:建立了以葡萄糖为对照品,紫外分光光度法测定参芎葡萄糖注射液中总糖的含量测定方法,且浓度在0.008-0.03 g/L范围内与吸光度线性关系良好,A=35.50C-0.041 80,r=0.999 3,平均回收率为103.1%,RSD为2.9%。结论:蒽酮-硫酸法测定参芎葡萄糖注射液中总糖含量,测定方法简便、准确可靠,可用于该制剂的质量控制。
Objective:To establish the method for the determination of total sugar in Shenxiong glu-cose injection.Methods:Adopting UV spectrophotometry and anthrone sulfur method,using glucose as reference to measure total sugar content of Shenxiong injection.Results:Established the method of using glucose as reference to test total sugar of Shenxiong glucose injection by UV spectrophotometry, and it showed a good linear relationship between the absorbance and the concentration between 0.008-0.03 g/L,A =35.50C -0.0418 0,r =0.999 3;the average recovery was 103.1%,RSD was 2.9%.Conclusions:The method is practical,accurate and reliable,can be used as quality control method of Shenxiong glucose injection.
出处
《贵阳医学院学报》
CAS
2015年第4期349-351,355,共4页
Journal of Guiyang Medical College
基金
国家科技支撑计划课题(2013BAI11B01)
贵州省科技重大专项项目[黔科合重大专项字(2011)6019号]
贵州省中药现代化专项项目[黔科合中药字(2013)5062号]
关键词
参芎葡萄糖注射液
总糖
紫外分光光度法
Shenxiong glucose injection
total sugar
ultraviolet spectrophotometry