气相色谱-质谱法测定鱼肉中丁香酚的残留

Determination of eugenol residue in fish meat by gas chromatography-mass spectrometry

研究建立一种鱼肉中丁香酚（eugenol）残留量的固相萃取-气相色谱-质谱检测方法.样品采用正己烷提取,硅胶柱净化,乙酸乙酯洗脱,气相色谱-质谱测定,内标法定量.丁香酚的响应值与其质量浓度在0.005～0.5 mg·L-1范围内的线性关系良好,相关系数为0.9998;10 ～200 μg·kg-1添加浓度的日内和日间平均回收率为80.6％～ 93.2％,相对标准偏差为3.47％～8.47％;检出限为5μg·kg-1,最低定量限为10 μg·kg-1.该方法适用于鱼肉中丁香酚残留的检测.

Gas chromatography -mass spectrometry coupled with solid -phase extraction was devel- oped for determination of eugenol residue in fish meat. The eugenol was extracted with n - hexane, purified by silicagel column and eluted with ethyl acetate, then analyzed by gas chromatography - mass spectrometry（ GC- MS）. The linear range was 0. 005 -0.5 mg · L-1 and its correlation coefficient was 0. 999 8. When spiked within 10 - 200 μg·kg-1 , the average recoveries of intra - day and inter - day were 80.6% - 93.2% , and their relative standard deviations were 3.47% - 8.47%. The limits of detection and quantitation were 5 μg·kg-1 and 10 μg·kg-1 respectively. This method can be applied to the determination of eugenol residues in fish meat.