不同剂量长双歧杆菌对抗生素诱导大鼠肠道菌群失调的疗效观察

The efficacy of different doses of Bifidobacterium longum on rat intestinal microbiota dysbiosis induced by antibiotic

目的观察不同剂量长双歧杆菌对抗生素诱导大鼠腹泻及肠道菌群失调的调节作用。方法按体表面积予头孢曲松钠钠腹腔注射诱导大鼠腹泻及肠道菌群失调,然后灌胃不同剂量的长双歧杆菌菌粉,观察治疗后大鼠肠道菌群数量、B/E值和血清内毒素的变化。结果予头孢曲松钠钠处理后,大鼠肠道内双歧杆菌数量明显减少,肠球菌异常增殖,B/E值明显下降,血清内毒素升高,与对照组相比差异有统计学意义(P<0.05)。高倍剂量治疗组肠道内细菌数量恢复程度及B/E值改善程度显著优于低倍剂量组。长双歧杆菌各治疗组的血清内毒素含量无明显改善,与自然恢复组之间无差异。结论头孢曲松钠可诱导大鼠肠道菌群失调。高倍剂量长双歧杆菌对头孢曲松钠所致肠道菌群失调效果更佳,短期应用不能明显改善头孢曲松钠诱发的内毒素血症。

Objective To observe the regulation of different doses of Bifidobacterium longum on rat intestinal microbiota dysbiosis induced by antibiotic. Methods The rats were intraperitoneally injected Ceftriaxone according to body surface area to induce diarrhea and intestinal Bifidobacterium longum. The changes of intestinal flora, microbiota dysbiosis, and then fed different doses of B/E value and endotoxin after treatment were observed. Results After intraperitoneal injection of ceftriaxone, the number of Bifidobacterium in the rat intestine signifi- cantly reduced, Enterococcus abnormally proliferated, B/E values obviously decreased and serum endotoxin elevated. Compared with the control group, the difference was significant （ P 〈 0. 05 ）. The recovery of intestinal bacteria and the improvement of B/E value were significantly better in high-dose group than in low-dose group. The serum endotoxin were not obviously reduced in each treatment group, and there was no difference when comparing with the natural recovery group. Conclusion Ceftriaxone can induce rat intestinal microbiota dysbiosis. High dose of Bifidobacterium longum are more effective in adjusting the alteration of intestinal microbiota induced by ceftriaxone. However, ceftriaxone related endotoxemia cannot be significantly improved in short term.