水稻抽穗期QTL定位及遗传分析

QTL Mapping and Genetic Analysis of Heading Date in Rice

以南粳35和N22杂交得到的F2群体为研究对象,通过构建遗传连锁图谱,对控制水稻抽穗期的QTL进行定位。利用Win QTLcart2.5软件共检测到7个控制水稻抽穗期的QTLs;分别位于第2、3、5、6、10和12染色体上,其中在第3染色体上检测到2个QTLs,每个QTL能够解释4.33%-18.12%的表型变异,其中q Hd-3-1对抽穗期表型贡献率最大。利用QTLNetwork2.0在第3和第5染色体上检测到有1对上位性QTLs,对表型的贡献率只有2.17%。进一步利用N22/南粳35//南粳35高代回交群体对q Hd-3-1功能进行验证,结果证明在南粳35背景下q Hd-3-1能明显缩短抽穗6 d,进一步证明q Hd-3-1是N22中控制抽穗期的一个主效QTL。

Using F2 population derived from N22 and Nanjing35 as the research object, the QTL contreling heading date were mapped through the construction of a genetic linkage map. Seven putative QTL were identified on chromosomes 2, 3, 5,6, 10, 12 using WinQTL cart2.5, two QTL were detected on chromosomes 3. These QTL could explain 4.33 % - 18.12 % trait variation, qHd-3-1 had the major effect in heading date. A pair of epistatic QTL were detected on chromosomes 3 and 5, only explained 2.17 % trait variation. Further, the effect of qHd-3-1 were confirmed using N22/Nanjing35//Nanjing35 AB-populations, the result showed that qHd-3-1 could significantly shorten heading 6 d than background parent Nanjing35, further proved that qHd-3-1 was a major QTL controlling heading date in N22.