基于16S rRNA基因序列的11个假眼小绿叶蝉地理种群遗传分化研究

Genetic Differentiation of Empoasca vitis( Gthe)( Hemiptera:Cicadellidae) among Eleven Populations Based on 16S rRNA Sequence

假眼小绿叶蝉Empoasca vitis（Gthe）是我国危害最为严重的茶园害虫。本研究对假眼小绿叶蝉11个地理种群的101个个体的16S rRNA序列片段进行了扩增和测序,以此分析11个地理种群间的遗传多样性、分子变异和种群遗传结构。结果表明：假眼小绿叶蝉16S rRNA基因序列片段全长493 bp,A＋T含量为77%。101个个体中共检测出变异位点11个,形成单倍型13个,单倍型进化网络关系显示四川群体具有的单倍型与原始单倍型（H2）距离较远。群体单倍型多样性指数为0.68812,种群内部单倍型多样性在0-0.80556之间,且总群体和各地理种群的中性检验结果均为不显著,表示假眼小绿叶蝉进化符合中性模型,其种群较为稳定。AMOVA分子变异分析结果表明假眼小绿叶蝉的遗传分化主要源于种群内部。

Empoasca vitis（ Gthe） is a most serious pest in tea plantation. In this study,we sequenced 16 S rRNA gene partial sequence for101 individuals in 11 geographical populations of E. vitis. Based on them,we investigated genetic diversity,molecular variation and population genetic structure for the 11 populations. The 16 S rRNA partial sequence is 493 bp long with a 77 % A ＋ T-content. 11 variable sites and 13 haplotypes were obtained,and haplotype network showed that haplotypes in Sichuan province are far from original haplotype（ H2）.The haplotype diversity index was 0. 68812 of total population,ranging from 0 to 0. 80556 within each population. The Tajima＇s D test for all populations or each single population did not reach significant level,it infered that the evolution and the abundance of E. vitis remained on quantitative level and fit well with the neutral model. AMOVA results demonstrated that genetic differentiation of E. vitis mainly occurred within population.