生姜内生溶磷细菌遗传多样性及溶解Al-P和Fe-P能力研究

Genetic Diversity of Phosphorous Dissolving Ginger Endophytic Bacteria and Their Al-P and Fe-P Dissolving Ability

开展生姜内生溶磷细菌的遗传多样性及溶磷能力研究,有助于获得新的溶磷微生物,促进土壤磷素转化。本文从采自重庆、四川雅安和眉山及贵阳等地生姜样品中分离得到32株内生溶磷细菌,采用BOX-PCR对其遗传多样性进行分析,进而研究供试菌株溶解Al-P和Fe-P的能力。BOX-PCR分析表明,供试菌株具有丰富的遗传带谱,聚类分析中,供试菌株在55%聚为一群;在70%的相似水平处,供试菌株分为6个BOX遗传群。在添加2 g/L Al-P和1 g/L Fe-P的液体培养基中,供试菌株溶解Fe-P能力高于溶解Al-P,有效磷含量分别为1.14-9.95和2.98-55.42μg/m L。其中,QW40溶磷效果最好,发酵液有效磷含量分别为9.95和55.42μg/m L。供试菌株对Al-P和Fe-P溶磷量与发酵液的p H值呈显著负相关关系。

The experiment was designed to study the genetic diversity of phosphate-dissolving endophytic bacteria isolated from ginger rhizome and to evaluate their Al-P and Fe-P dissolving ability,which helps to explore the new phosphate-solubilizing microorganisms for promotion of soil phosphorus nutrition uptake. A total of 32 phosphate-dissolving endophytic bacteria were isolated from ginger rhizome collected from Chongqing,Ya＇an and Meishan in Sichuan Province,Guiyang in Guizhou Province,and the genetic diversity of these isolates was analyzed by BOX-PCR; then the Al-P and Fe-P dissolving ability of 16 selected isolates was determined as well. The results showed that the genetic diversity was existed; based on BOX-PCR analysis,all the strains formed one group at the level of 55 %,and six BOX genotypes were clustered at the level of 70 % similarity. The results also revealed that these strains had higher Fe-P dissolving ability than that of Al-P. The available phosphorus（ AP） was 1. 14-9. 95 and 2. 98-55. 42 μg·mL-1in the liquid culture when adding 2 g / L Al-P and 1 g / L Fe-P,respectively; after inoculation of QW40 and incubation,the AP in liquid culture was 9. 95 and 55. 42 μg · mL-1,which suggested that QW40 had the strongest P-releasing ability. The results also indicated that the AP in liquid culture was significantly negative correlation to the culture media pH value.