连翘酯苷不同给药途径对副流感病毒致家兔发热模型的影响

Studies of different administration routes of Forsythiaside on rabbit fever models induced by parainfluenza virus

目的:考察连翘酯苷不同给药途径对副流感病毒致热家发热模型解热作用的影响。方法:将连翘酯苷分别以静脉注射和口服灌胃的途径对副流感病毒感染家兔发热的模型进行治疗性给药,静脉注射途径观察给药后0.5h、1h、2h的解热作用,口服灌胃途径观察给药后1h、2h、3h的解热作用,并检测各组动物下丘脑中发热介质蛋白激酶C(PKC)和环磷酸腺苷(c AMP)的含量。结果:静脉注射连翘酯苷(60mg/kg、120mg/kg)0.5h后家兔体温差值为(0.72±0.26)、(0.83±0.24)℃,1h后体温差值为(0.82±0.23)、(0.92±0.34)℃,灌胃给予连翘酯苷(60mg/kg、120mg/kg)后1h家兔的体温差值为(0.43±0.42)、(0.45±0.42)℃,与模型比较均具有显著差异,起效剂量为60mg/kg。静脉注射连翘酯苷(30mg/kg、60mg/kg、120mg/kg)均能降低家兔下丘脑中环磷酸腺苷含量,分别为(13.92±9.11)、(16.47±7.98)、(19.75±5.04)nmol/L,灌胃给予相应剂量的连翘酯苷则对发热介质无影响。结论:连翘酯苷静脉注射和口服灌胃均能明显降低副流感病毒感染后家兔的体温,且静脉注射的解热作用要优于口服灌胃,其解热作用机制可能与下调家兔下丘脑组织中的c AMP含量有关。

Objective： To investigate the antipyretic effect of different administration routes of Forsythiaside and its mechanism. Methods： The fever model was induced by injecting parainfluenza virus into rabbits. Body temperature of rabbits was measured at 0. 5h,1h,2h after injecting Forsythiaside intravenously and 1h,2h,3h after administrating Forsythiaside by gastric gavage. Then,both PKC and c AMP activity in hypothalamus were measured. Results： The body temperature of rabbits was reduced at 0. 5h,1h after intravenous injection of Forsythiaside and at 1h after intragastric administration. Starting effective dose of Forsythiaside was 60 mg / kg. The content of c AMP in the hypothalamus was reduced in fever rabbits that injected Forsythiaside（ the doses were 30 mg / kg,60 mg / kg,120 mg / kg）. Conclusion： Forsythiaside could subside the fever in rabbits induced by parainfluenza virus whatever intravenous injection or intragastric administration.