大黄总蒽醌对人肾小管上皮细胞毒性作用及相关机制研究

Cell toxic effect of total anthraquinone in Rhubarb on human renal tubular epithelial cells and the related mechanism

目的:探讨在大黄总蒽醌对人肾小管上皮细胞毒性作用及相关机制。方法:以MTT法检测细胞毒性;CPE观察细胞形态学改变,流式细胞仪检测细胞周期、凋亡率。结果:大黄总蒽醌对HK-2细胞增殖有直接的抑制作用,呈一定的量效关系,并使细胞形态发生相应改变。大黄总蒽醌能抑制HK-2细胞S期向G2/M期的转化,引起细胞凋亡,尤其在较低剂量(3.75 mg/L)下就可以引起晚期凋亡/坏死,当药物浓度在30 mg/L以下剂量时,其抑制细胞增值作用和对细胞周期的阻滞作用相对较弱,在30mg/L增加到60 mg/L时的过程中,抑制细胞增值和细胞周期的阻滞作用增加明显,甚至出现了明显的晚期凋亡/坏死,高浓度时凋亡率大于50%。结论:大黄总蒽醌对HK-2细胞具有一定的毒性作用,其毒性作用可能与影响细胞周期和凋亡相关。

Objective： To investigate the cell toxic effect of total anthraquinone in Rhubarb on human renal tubular epithelial cells and its related mechanism. Methods： The cell toxic effect,the change of cell morphology,cell cycle and apoptotic analysis were measured by MTT assay,CPE and flow cytometry（ FCM）,respectively. Results： Rhubarb total anthraquinone HK-2 cell proliferation direct inhibitory effect showed a dose-effect relationship,and morphological changes accordingly. Rhubarb can inhibit the conversion of total anthraquinone HK-2 cells in S phase to G2 / M phase and induce apoptosis,especially in the lower dose（ 3. 75 mg / L） can cause lower late apoptosis / necrosis,when the drug concentration in 30 mg / L or less when the dose,and its role in inhibiting cell proliferation and cell cycle arrest in relatively weak,at30 mg / L to 60 mg process / L,in inhibiting cell proliferation and cell cycle arrest in effect increased significantly,and even there is a clear late apoptosis / necrosis,apoptosis rate at high concentrations greater than 50%. Conclusion： Cell toxic effect of total anthraquinone in Rhubarb on HK-2 was existent and maybe related to the effect of the cell cycle and apoptosis.