β-咔啉类生物碱对人胃癌SGC-7901细胞增殖、凋亡的影响及机制探讨

Exploration on influence and mechanism of β-carbolines alkaloid on human gastric cancer SGC-7901 cell proliferation and apoptosis

目的观察β-咔啉类生物碱对体外培养的人胃癌SGC-7901细胞增殖及凋亡的影响,并探讨其作用机制。方法不同浓度β-咔啉类生物碱处理人胃癌SGC-7901细胞48 h后,检测细胞增殖、凋亡情况,并以荧光定量聚合酶链反应(RTPCR)和蛋白印迹法(Western blot)分别测定细胞中抑癌基因PTEN、蛋白激酶B(AKT)基因mRNA及其蛋白表达。结果不同浓度的β-咔啉类生物碱均能抑制人胃癌SGC-7901细胞的增殖,其中40μg/m L浓度的抑制效果较其他浓度及5-Fu干预组更显著(P<0.01),并可诱导细胞凋亡。不同浓度β-咔啉类生物均可引起PTEN mRNA和蛋白表达增加,AKT mRNA和蛋白表达减少,其中40μg/m L浓度的调节效果较其他浓度及5-Fu干预组更显著(P<0.01)。结论β-咔啉类生物碱可抑制人胃癌SGC-7901细胞增殖,诱导凋亡,其作用机制可能是通过上调PTEN及下调AKT蛋白的表达实现。

Objective To observe the influence of β-carbolines alkaloid on in-vitro cultured human gastric cancer SGC-7901 cell proliferation and apoptosis and to explore its functional mechanism. Methods Human gastric cancer SGC-7901 cells were treated with different-concentration β-carbolines alkaloid for 48 hours so as to detect the cell proliferation and apoptosis,and fluorescence quantitative polymerase chain reaction（RT-PCR） and Western blot analysis were conducted to detect the expressions of anti-oncogene PTEN mRNA and protein kinase B（AKT） gene mRNA as well as their proteins in cells. Results Different-concentration β-carbolines alkaloid could inhibit the proliferation of human gastric cancer SGC-7901 cells,in which the efficacy treated with 40 μg / m L was evidently higher than other concentrations and 5-Fu intervention group（P〈 0. 01） and it could also induce cell apoptosis. Different-concentration β-carbolines alkaloid could increase the expressions of PTEN mRNA and its protein and reduce the expressions of AKT mRNA and its protein,in which the efficacy of treatment with 40 μg / m L was evidently higher than other concentrations and 5-Fu intervention group（P〈 0. 01）. Conclusion β-carbolines alkaloid can inhibit the human gastric cancer SGC-7901 cell proliferation and induce cell apoptosis,and the functional mechanism may be achieved by up-regulating PTEN protein and down-regulating AKT protein.