痤疮丙酸杆菌对米诺环素的敏感性研究

Sensitivity of Propionibacterium acnes to minocycline

目的:明确痤疮丙酸杆菌对米诺环素的敏感性。方法:收集236例寻常性痤疮患者并给予米诺环素口服。治疗前采用需氧培养和厌氧培养的方法,对患者皮损进行病原菌分离。治疗前后利用抗菌药敏纸片对痤疮丙酸杆菌进行药物(米诺环素、四环素、罗红霉素和替硝唑)耐药性检测。肉汤稀释法测定米诺环素的最低抑菌浓度。结果:病原菌分离结果示:前3位病原菌分别为痤疮丙酸杆菌(32.45%)、金黄色葡萄球菌(19.95%)和表皮葡萄球菌(13.83%);治疗前痤疮丙酸杆菌对米诺环素、四环素、罗红霉素和替硝唑的耐药率分别为0、20.93%、83.72%和98.8%,治疗后为0、47.22%、86.11%和100%。米诺环素对痤疮丙酸杆菌的MIC_(50)和MIC_(90)分别为0.125μg/mL和0.25μg/mL。结论:痤疮丙酸杆菌对米诺环素仍然敏感。

Objective：To determine the sensitivity of Propionibacterium acnes （P. acnes） to minocycline （ MNO） . Methods：Two hundred and thirty-six patients with acne vulgaris were treated with MNO. Pathogens from the patients were isolated and identified through the method of aerobic and anaerobic cultivation before the treatment. The drug-resistance of P. acnes to minocycline （MNO）, tetracycline （TCY）, roxithromycin （ROX） and tinidazole （TIN） was tested by disk agar diffusion method before and after the treatment. The minimum inhibiting concentration （ MIC） of MNO to P. acnes was detected by broth dilution method. Results：The main pathogens were P. acnes （32.45%）, Staphylococcus aureus（19.95%） and Staphylococcus epidermi-dis （13.83%）. The drug-resistance of P. acnes to MNO, TCY, ROX and TIN before the treatment was 0, 20.93%, 83.72% and 98. 8% and after the treatment was 0, 47. 22%, 86. 11% and 100%. The MIC50 and MIC90 of MNO to P. acnes were 0.125μg/mL and 0.25μg/mL. Conclusion：P. acnes still remain sensitivity to MNO.