植物组织培养寻求最佳培养基的方法研究

Research Methods in Study on Optimum Medium in Plant Tissue Culture

植物组织培养技术在植物的个体发育、器官再生和生理代谢等理论研究中已成为一种重要手段,最佳培养基的寻找,是组培成败的关键。从基本培养基和植物生长调节剂入手,植物组织培养最佳培养基的研究方法由传统的定性研究转向现代的定量研究,加快寻求最佳培养基的步伐。

Over the past few decades, plant tissue culture has evolved into one of the major research tools in many fields such as ontogeny, organogenesis and physiological metabolism, etc. In practice, it has reached a level of sophistication where, for some species, its adaptation to large-scale industrial use has become possible, especially for quick reproducing elite seedlings. Though great progress has been achieved most of successful euhuring procedures in vitro were base on experiences rather than on theories and concepts. This paper reviewed recent advances in search of media that would be suitable to plant regenerations in plant tissue culture. With focusing on the methods of optimizing plant growth regulate, macro elements and nutrition components at different culturing stages, it is hope that improving the research means for media would make steps from traditional experiments into more intentional and help to development of seeking optimal medium components.