IL-1β对人牙龈成纤维细胞在不同钛板表面表达炎性因子的影响

Effect of IL-1β on inflammatory factors synthesis of human gingival fibroblasts attached to different titaniums

目的 :探讨白介素1β对不同钛板表面牙龈成纤维细胞分泌炎性因子的影响。方法 :将牙龈成纤维细胞接种在3种不同表面结构组分的钛板上,在白介素1β的刺激下,在3、6、24 h分别用聚合酶链反应(PCR)检测炎性因子白介素6及白介素8的表达量。采用SPSS13.0软件包对数据进行统计学分析。结果:在白介素1β的诱导下,牙龈成纤维细胞对炎性因子(IL-6、IL-8)的表达量明显增加;纯钛与钛合金的钛板比表面氮化处理的钛板产生炎性因子(IL-6、IL-8)的表达量较少。结论 :白介素1β能刺激炎性因子的高表达;纯钛和钛合金IL-6、IL-8表达量较少。采用纯钛和钛合金种植体,可减少种植体周围炎的发生率,提高种植成功率。

PURPOSE： To evaluate the effects of IL-1β on inflammatory factors synthesis of human gingival fibroblasts on different surfaces of titaniums in vitro. METHODS： Human gingival fibroblasts were cultured on 3 different kinds of titaniums. The cells were stimulated with IL-1β, and the IL-6 and IL-8 production was detected by polymerase chain reaction （PCR） after 3, 6 and 24 h. The date was statistically analyzed with SPSS13.0 software package. RESULTS： A significant increase of the expression of gingival fibroblasts to inflammatory factors （IL-6, IL-8） after IL-1β was noted; PCR results of the production of inflammatory factors in gingival fibroblasts with the intervention of IL-1β showed that the production of inflammatory factors （IL-6, IL-8） on titanium and titanium alloy were less than that on the titanium nitride. CONCLUSIONS： IL-1β can stimulate high expression of inflammatory factors; The production of IL-6 and IL-8 on titanium and titanium alloy are less, suggesting that implant made of titanium and titanium alloy can reduce the incidence of peri-implant inflammation, and improve the successful rate of implantation.