口腔鳞癌组织中Ki-67和p53蛋白的表达

Expression of Ki-67 and P53 protein in oral squamous cell carcinoma and its clinical significance

目的 :探讨口腔鳞癌组织中Ki-67和p53蛋白的表达及其与临床病理特征的关系。方法 :采用免疫组织化学S-P法对10例正常口腔黏膜组织、16例口腔白斑(OLK)组织、48例口腔鳞癌(OSCC)组织中的Ki-67和p53蛋白表达进行检测,结合患者临床病理资料进行分析,使用SPSS17.0软件包对数据进行统计学处理。结果:Ki-67蛋白在正常口腔黏膜组织、口腔白斑和口腔鳞癌组织中的阳性表达率分别为30.0%、56.3%和79.2%;p53的阳性表达率分别为0.0%、43.8%和70.8%,Ki-67和p53在正常黏膜组与口腔白斑和口腔鳞癌组差异均具有显著性(P<0.05);Ki67蛋白在口腔鳞癌组织中的表达与肿瘤的临床分期、分化程度、有无淋巴结转移有关(P<0.05),p53蛋白的表达与肿瘤的分化程度有关(P<0.05);Ki-67和p53蛋白在口腔鳞癌组织中的表达呈正相关(P<0.05)。结论:Ki-67和p53蛋白在口腔鳞癌组织中高表达,可能在口腔鳞癌的发生、发展过程中起着重要作用。

PURPOSE： To investigate the clinical and pathological features and its relationship with the expression of Ki- 67 and p53 protein in oral squamous cell carcinoma. METHODS： Immunohistochemical SP staining method was used to quantify the protein expression levels of Ki-67 and p53 protein in 10 cases of normal oral mucosa, 16 cases of oral leukoplakia （OLK） tissue, and 48 cases of oral squamous cell carcinoma. The relationship of the expression of Ki-67 and p53 protein to clinical and pathological data was analyzed, and SPSS17.0 software package was used for statistical analysis. RESULTS： The positive expression rate of Ki-67 protein in normal＇ oral mucosa, oral leukoplakia and oral squamous cell carcinoma was 30%, 56.3% and 79.2%, respectively; The positive expression rate of p53 was 0%, 43.8%, and 70.8%, respectively; Ki-67 and p53 expression had significant difference among normal oral mucosa, oral leukoplakia and oral squamous cell carcinoma （P〈0.05）; The expression of Ki-67 protein was significantly elevated with tumor stage, differentiation and cervical lymph node metastasis （P〈0.05）; The expression of p53 protein was significantly related to the degree of tumor differentiation （P〈0.05）; The expression of Ki-67 and p53 was positively correlated in oral squamous cell carcinoma （P〈0.05）. CONCLUSIONS： The high expression of Ki-67 and p53 protein in oral squamous cell carcinoma tissues may play an important role in the development of oral squamous cell carcinoma.