土槿皮乙酸对胰腺癌AsPC-1细胞增殖和凋亡的影响

Effects of pseudolaric acid B on the proliferation and apoptosis in pancreatic carcinoma cell line AsPC-1

目的:观察土槿皮乙酸(pseudolaric acid B,PAB)对胰腺癌AsP C-1细胞增殖和凋亡的影响。方法:体外培养胰腺癌AsP C-1细胞,用不同浓度PAB作用不同时间后,MTT法检测PAB对AsP C-1细胞增殖的影响,流式细胞仪检测细胞凋亡率。结果:MTT结果表明PAB以时间和剂量依赖的方式抑制AsP C-1细胞生长,流式结果显示8.0μmol/L PAB处理AsP C-1 24小时后,凋亡率由对照组的8.19%升高至30.86%,差异有统计学意义(P<0.05),表明PAB可诱导胰腺癌细胞凋亡,呈剂量依赖性。结论:土槿皮乙酸以时间和剂量依赖的方式抑制胰腺癌AsP C-1细胞增殖并诱导其凋亡。

Objective：To examine the effects of pseudolaric acid B（PAB）on the proliferation and apoptosis of human pancreatic carcinoma cell line ASPC - 1. Methods：ASPC - 1 cells cultured in vitro were treated with various concentrations（0. 5,1. 0,2. 0,4. 0,8. 0μmol/ L）of PAB at various intervals. Growth inhibition was analyzed by 3 -（4,5 - dimethylthiazol - 2 yl）- 2,5 - diphenyltetrazolium bromide（MTT）assay. Apoptotic cells were detected by flow cytometry. Results：Pseudolaric acid B inhibited the proliferation of ASPC - 1 cells in a time - and dose - de-pendent manner. The inhibition ratio was（4. 53 ± 3. 32）% after the cells were treated with 0. 5μmol/ L PAB for 24h, and the ratio was（52. 22 ± 1. 71）% after the cells were treated with 8. 0μmol/ L PAB for 72h. ASPC - 1 cells treated with 8. 0μmol/ L PAB for 24h showed significantly enhanced apoptosis as compared with the control cells（8. 19% vs 30. 86% ,P 〈 0. 05 ）.Conclusion：PAB can dramatically suppress the ASPC -1 cell growth and induce cell apoptosis.