ER-α36在乳腺癌组织中的表达及临床意义

Expression and clinical significance of ER-α36 in breast cancer

目的:研究ER-α36(estrogen receptor-alpha 36)在乳腺癌组织中的表达状态及在乳腺癌发生、发展和临床预后中的作用。方法:选取河南省肿瘤医院乳腺科及郑州大学第一附属医院乳腺科2010年3月-2013年3月间653例乳腺癌组织,用RT-PCR技术和免疫组化技术检测组织中ER-α36、ER-α66、PR和Her-2的表达,分析ER-α36的表达与上述指标及临床病理特征之间的关系。结果:ER-α36的总体表达率是40%(261/653)。在Her-2阳性组织中的表达率63%(78/124),显著高于阴性组35%(183/529),P<0.05。在ER-α66/PR/Her-2三阴性组织中的表达率66%(69/104),显著高于非三阴性组35%(192/549),P<0.05。在ER-α66阳性和阴性样本中及在PR阳性和阴性样本中的表达率,差异没有统计学意义(P>0.05)。在III+IV期乳腺癌组织中的阳性表达率54%(165/305),明显高于I+II期28%(96/348),P<0.05。在淋巴结转移阳性乳腺癌组织中的表达率55%(184/335),显著高于无转移组织24%(77/318),P<0.05。结论:ER-α36可能在乳腺癌的发生、发展及淋巴结转移过程中具有重要作用,并与Her-2的表达、乳腺癌分期及淋巴结转移有关,有望成为新的肿瘤标记物及临床诊治的新靶点。

Objective：To study the expression status of ER - α36 in breast cancer tissue and its roles in the oc-currence,development,clinical prognosis of breast cancer. Methods：All 653 cases with breast cancer were selected from March 2010 to March 2013 in the Breast Department of Henan Cancer Hospital and the First Affiliated Hospital of Zhengzhou University. RT - PCR and immunohistochemistry was used to detect the expression of ER - α36,ER -α66,PR and Her - 2. The relationship between the expression of ER - α36,ER - α66,PR and Her - 2 and the patho-logical character was analyzed. Results：The expression rate of ER - α36 in all cases was 40%（261 / 653）. The ex-pression rate of ER - α36 in Her - 2 positive tissues was 63%（78 / 124）,which was significantly higher than that in the Her - 2 negative group（35% ,183 / 529）（P 〈 0. 05）. The expression rate of ER - α36 in ER - α66 / PR/ Her - 2 negative tissues was 66%（69 / 104）,which was significantly higher than that in the non - three - negative group （35% ,192 / 549）（P 〈 0. 05）. The differences of ER - α36 expression rate between ER - α66 positive samples and negative samples or between PR positive and negative samples showed no statistical significance（P 〉 0. 05）. The ex-pression rate of ER - α36 in stage III ＋ IV breast cancer tissue was 54%（165 / 305）,which was significantly higher than that in stage I ＋ II breast cancer tissue（28% ,96 / 348）（P 〈 0. 05）. The expression rate of ER - α36 in breast cancer tissue with lymph node metastasis was 55%（184 / 335）,which was significantly higher than that in breast cancer tissue without lymph node metastasis（24% ,77 / 318）（P 〈 0. 05）. Conclusion：The results indicate that ER- α36 may play an very important role in the occurrence,development and lymph node metastasis of breast cancer, and be associated with the expression of Her - 2,breast cancer staging and lymph node metastasis. ER - α36 is expec-ted to become a new tumor marker in clinical diagnosis and treatment target.