食管鳞癌中GPX3基因的甲基化特征及其临床意义

Methylation characters of GPX3 gene in esophageal squamous cell carcinoma and its clinical significance

目的:采用将食管鳞癌组织和癌旁组织相对比,并将食管鳞癌患者的血浆与正常人的血浆相对比的策略,检测GPX3的甲基化状态和频率。结合患者的临床病理特征,分析GPX3基因甲基化在食管鳞癌中的意义。方法:收集42例食管鳞癌组织标本和相对应的癌旁组织标本,同时收集42例食管鳞癌患者和50例正常人的血浆标本;应用甲基化特异性PCR(MSP)结合琼脂糖凝胶电泳检测GPX3在组织和血浆中的甲基化情况,对比分析。结果:GPX3在肿瘤组织中的甲基化频率为54.8%,显著高于癌旁组织9.5%(P<0.001)。GPX3在肿瘤患者血浆中的甲基化频率为40.5%,在正常对照组的血浆中没有检测到甲基化。组织中的甲基化频率在病理分期为T3组和N2组分别显著高于病理分期为T1-T2组和N0-N1组。血浆中的甲基化频率在病理分期T3组明显高于病理分期T1-T2组。结论:GPX3在食管鳞癌组织的甲基化频率高于癌旁组织,在食管鳞癌患者血浆中的甲基化频率显著高于正常人,且临床分期越晚的甲基化频率越高。

Objective ：To detect GPX3 gene methylation frequency difference between esophageal squamous cell carcinoma（ESCC） tissues and control normal tissues, between ESCC patients＇plasma and healthy volenteers＇plasma, and to analyze the correlation with elinico - pathological parameters. Methods： We collected esophageal squamous cell carcinoma tissues（n =42 eases） and adjacent surrounding normal tissues（ n = 42 cases）,plasma of the esophageal squamous cell carcinoma（ n = 42 cases） and plasma of the healthy individuals （n = 50 cases）. We used methylation specific PCR（MSP） combined with agarose gel electrophoresis to detect the methylation status of the GPX3. Results： GPX3 frequency of methylation in tumor tissue was 54.8%, significantly higher than the＇adjaeent tissues which was 9.5%, the difference was statistically significant（ P 〈 0.001 ）. In the plasma of cancer patients GPX3 methylation frequency was 40.5% ,while the methylation of GPX3 in the plasma of healthy volunteers was not deteeted. Methylation of GPX3 in tissue was more frequently seen in patients with T3 or N2 than in patients with T1-2 or N0-1. Conclusion： The methylation frequency of GPX3 is higher in ESCC tissus than in control normal tissues, and higher in plasma of ESCC patients than in healthy volunteers. GPX3 methylation was more frequently seen in patients with more advanced disease.