胃腺癌患者外周血中CD4^+ T淋巴细胞表面抑制性分子PD-1的表达

Expression of inhibitory molecule PD-1 on CD4^+ T lymphocytes in peripheral blood of gastric adenocarcinoma patients increased

目的:检测胃腺癌患者外周血中程序性死亡分子1(programmed death-1,PD-1)在CD4+T淋巴细胞上的表达水平,探讨其与胃腺癌的关系。方法:选取未经放、化疗或外科手术治疗的胃癌患者57例、疾病对照22例与健康对照20例,取新鲜抗凝血经流式细胞术(flowcytometry,FCM)检测抑制性分子PD-1的百分比例;同时对胃腺癌患者进行TNM分期比较。结果:胃腺癌患者外周血中PD-1在CD4+T淋巴细胞上的表达水平(10.91±4.36)%比疾病对照(8.14±3.65)%和健康对照(7.37±3.06)%显著升高,Ⅲ-Ⅳ期胃腺癌患者的CD4+PD-1+T细胞的百分比例(11.9±3.85)%显著高于Ⅰ-Ⅱ期(9.08±3.13)%。结论:抑制性分子PD-1在胃腺癌外周血中CD4+T淋巴细胞表面表达增加,并随胃腺癌进展而升高,提示CD4+PD-1+T淋巴细胞与胃腺癌的发生发展相关。

Objective： To detect the expression level of PD - 1 on CD4^＋ T lymphocytes in peripheral blood of gastric adenocarcinoma patients and investigate the relationship with progression of gastric adenocarcinoma. Methods： Anticoagulated fresh bloods of 57 gastric adenoearcinoma patients, 22 disease controls and 20 healthy controls were studied. The expression level of inhibitory molecule PD - 1 was assayed by flowcytometry （FCM）. Results ： The percentage of CD4 ^＋ PD - 1 ^＋ T cells were significantly higher in peripheral blood of gastric adenocarcinoma patients than in disease controls and healthy controls ; The ratio of CD4^＋ PB - 1 ^＋ T ceils significantly increased in patients with stages Ⅲ and Ⅳ gastric adenocarcinoma as compared with patients with stages Ⅰ and Ⅱ gastric adenocarcinoma. Conclusion： The increased expression of inhibitory molecules PD - 1 on CD4^＋ T lymphocytes in peripheral blood of gastric adenocarcinoma patients, and increased with gastric adenocarcinoma progression, indicating that CD4^＋PD - 1 ^＋ T lymphocytes correlates with the occurrence and progression of gastric adenocarcinoma.