缺氧预处理对颅脑损伤大鼠NF-κB表达及神经元活性的影响

Effect of hypoxic preconditioning on NF-κB expression and neuronal activity in rats after traumatic brain injury

目的探讨缺氧预处理(HPC)对颅脑损伤大鼠NF-κB表达及神经元活性的影响。方法将雄性SD大鼠,随机分为对照组(Con组)、缺氧预处理组(HPC组)、外伤组(TBI组)、缺氧预处理+外伤组(HPCT组)。采用RT-q PCR、Western blotting测定挫伤区NF-κB mRNA和蛋白表达,免疫组化Neu N染色法测定神经元活性变化情况。结果 TBI组和HPCT组NF-κB mRNA 3 h-1 d明显升高,NF-κB蛋白3 h-7 d明显升高,Neu N 3 h-14 d明显降低(P<0.05)。HPCT组NF-κB mRNA 3 h-1 d及NF-κB蛋白3 h-7 d均低于TBI组,而Neu N表达3 h-7 d高于TBI组(P<0.05)。结论缺氧预处理在一定时间窗内抑制NF-κB表达可能对神经元起保护作用。

Objective To explore the effect of hypoxic preconditioning on neuronal activity in traumatic brain injury rats, and to research the influence of nuclear factor-κB （NF-κB） on neuronal activity.Methods Male rats were randomly divided into control group （ Con ）, hypoxic preconditioning group （HPC）, traumatic brain injury group （TBI） and traumatic brain injury after hypoxic preconditioning group （ HPCT ） .RT-qPCR and Western blotting were used to detect the expression of NF-κB, Immunohistochemistry was used to measure the average optical density of NeuN-positive.Results In the TBI and HPC groups, the mRNA levels of NF-κB at 3 h, 6 h, 12 h, 1 d and the expression of NF-κB at 3 h, 6 h, 12 h, 1 d, 3 d, 7 d were all significantly increased （ P〈0 .05 ） .The expression of NeuN in TBI and HPC groups were significantly decreased during the period from 3 h to 14 d（P〈0.05）.In the HPCT group, the mRNA levels of NF-κB at 3 h, 6 h, 12 h, 1 d and the expression of NF-κB at 3 h, 6 h, 12 h, 1 d, 3 d, 7 d were lower than TBI group , however , the expression of NeuN were higher than TBI group at the same period with NF-κB（P〈0.05）.Conclusion Hypoxic preconditioning could protect neurons via inhibiting the expression of NF-κB in rats crotex after traumatic brain injury within a certain time window .