胶质瘤中过表达长链非编码RNA loc285194促进胶质瘤细胞凋亡

Over-expression of long non-coding RNA loc285194 in glioma promotes glioma cells' apotosis

目的探索上调长链非编码RNAloc285194对胶质瘤细胞系U87细胞生长的影响。方法荧光定量PCR验证lncRNA loc285194在胶质瘤样本以及U87细胞中的表达;构建过表达lncRNA loc285194的U87稳定细胞株;CCK8检测细胞增殖能力;流式细胞仪检测凋亡能力。结果 (1)lncRNA loc285194在胶质瘤样本中低表达且于肿瘤的恶性程度存在相关性;(2)上调lncRNA loc285194能抑制U87细胞的增殖;(3)上调lnc loc285194能促进细胞凋亡。结论在胶质瘤细胞中上调lncRNA loc285194能减慢胶质瘤细胞的生长,提示lncRNA loc285194可能成为一个新的治疗靶点。

Objective To investigate the long non coding RNA （ lncRNA） loc285194 expression levels in glioma and the role of lncRNA loc 285194 in human glioma u87 cell biological functions . Methods Quantitative real-time polymerase chain reactions for lncRNA-loc285194 expression were performed in 4 epilepsy brain tissues and 38 pairs of glioma tissues and adjacent normal tissues in our department .Over-expressed of lncRNA loc 285194 cell lines were constructed .Cell growth , and apoptosis were respectively tested by CCK 8 assay and flow cytometry .Western blot was also used to detected p53 protein.Result lncRNA loc285194 was significantly low-expressed in glioma tissues . Over-expressed lncRNA loc285194 inhabited human glioma U87 cells growth （ P 〈0.05 ） and promoted U87 cells apoptosis （ P 〈0 .05 ） .Conclusions lncRNA loc285194 is low-expressed in human glioma tissues . Over-expression lncRNA loc 285194 suppress glioma cells growth and promoted glioma cell apoptosis through inducing p 53 protein.It might be a new gene therapy target .