液相色谱-质谱法测定酒中甜蜜素的内标适用探讨

Applicability of internal standard in determination of cyclamate in wine by HPLC-tandem mass spectrometry

[目的]探讨液相色谱-质谱联用法测定酒中甜蜜素的内标适用性。[方法]酒样直接稀释后,采用液相色谱-质谱联用法测定,并分别用外标法和内标法对结果进行定量。[结果]2种定量方法的结果平均相差11.2%,最大相对相差为16.2%,而外标定量法的结果与国标方法 GB/T 5009.97—2003测定的结果一致。[结论]结果差异源于液质联用的基质效应及其内标化合物选择的不适。

Objective] To discuss the applicability of internal standard in determination of cycla-mate in wine by HPLC-tandem mass spectrometry . [ Methods] After dilution wine sample was deter-mined by HPLC-tandem mass spectrometry and quantified by external and internal standard methods respec-tively. [Results] The results by the 2 quantitation methods differed 11.2%in average, and the maxi-mum relative deviation was 16.2%.And the result by external standard quantitation method proved to be consistent with that by the international standard GB/T 5009.97-2003. [ Conclusion] The difference in results is thought to originate from matrix effect in HPLC-tandem mass spectrometry and the improper in-ternal standard compound applied .