华南8号木薯块根形成期叶绿体蛋白质组学分析

Proteomic Analysis of Chloroplast in Cassava Cultivar ‘South China 8' During the Tuberous Root Formation

以‘华南8号’木薯块根形成期叶绿体为材料,采用改进酚抽法提取总蛋白,通过一维和二维电泳,经Image Master分析,获得了木薯叶绿体的蛋白质表达谱,发现(397±31)个蛋白点;挖取2-DE凝胶上相对丰度较高的蛋白点275个,利用改进的酶解方法进行胶内酶解,经MALDI-TOF MS质谱鉴定,获得208个蛋白点,对应143种蛋白质,这些蛋白主要参与光合作用、光合生物碳固定、氧化还原调节、氨基酸代谢、蛋白质代谢和转运等途径。结果为后期从蛋白质组水平深入研究木薯叶绿体高光效机制提供一定参考。

The improved phenol method was used for total protein extraction from the chloroplast of forming stage tuberous root in cassava cultivar ‘South China 8＇（SC8）, and these proteins expressed profile were determined with1-DE and 2-DE. The obtained typical 2-DE gels were analyzed by Image Master software, and generated（397±31）protein spots. Two hundred and seventy five protein points with relatively high abundance were harvested and enzymolized using the improved enzyme solution method. Two hundred and eight proteins points were finally identified by MALDI-TOF MS, corresponding to 143 proteins, which were involved in photosynthesis, carbon fixation, redox balance regulation, amino acid metabolism, protein metabolism and transport, and so forth. This study acquired the information of cassava chloroplast proteins during the tuberous root formation, preliminarily analyzed these proteins＇ function, and provided an important fundamental technology for further proteomic research on the high photosynthetic efficiency mechanism of cassava cultivar.