LP17对大鼠肠巨噬细胞超微结构的影响

Effect of LP17 on the ultrastructure of intestinal macrophages of rats

目的:通过观察LP17对体外培养的大鼠肠巨噬细胞超微结构的影响,探讨LP17对激活的肠巨噬细胞的作用机制,寻找对肠黏膜屏障功能障碍的可能治疗方法。方法:体外分离、培养大鼠肠巨噬细胞分为对照组[未加脂多糖(LPS)和LP17处理],及实验组[包括LPS组(用LPS处理)和LPS+LP17组(用LPS及LP17处理)]。给药浓度为LPS 1 mg/L,LP17 0.1 mg/L,培养6 h后用0.25%胰酶消化收集细胞。Tecnai 12透射电镜观察实验组及对照组大鼠肠巨噬细胞的超微结构变化。结果:经药物处理后,电镜下观察,对照组为正常巨噬细胞,实验组中LPS组巨噬细胞内出现大量溶酶体,LPS+LP17组巨噬细胞出现凋亡小体。结论:LP17能促使被激活的巨噬细胞凋亡。

Objective：To investigate the effects of LP17 on the ultrastructure of intestinal macrophages of rats in vitro, explore the activated mechanism of intestinal macrophage and search for the treatment of intestinal mucosal barrier dysfunction. Methods：The intestinal macrophages of rat were separated and cultured in vitro, and divided into the control group, （ non-LPS and -LP17 ） and experiment group,which included the lipopolysacharide（ LPS） group（ treated with 1 mg/L of LPS） and LPS＋LP17 group（ treated with 1 mg/L of LPS ＋0. 1 mg/L of LP17）. Cells were digested by 0. 25% trypsin,and collected after 6 h of incubation. The ultrastructure of macrophages in all groups were observed using Tecnai 12 transmission electron microscopy. Results：The normal macrophages in control group,a large number of lysosomes in macrophages of LPS group and apoptotic bodies in macrophages of LPS＋LP17 group were found under electron microscopy after drug treatment. Conclusions：LP17 can induce the apoptosis in activated macrophages.