CD147和基质金属蛋白酶-9在肝细胞肝癌中的表达与微血管生成的相关性

The expressions of CD147 and matrix metalloproteinases-9 in hepatocellular carcinoma,and its correlations with clinical pathology and angiogenesis

目的：探讨细胞外基质金属蛋白酶诱导因子（CD147）和基质金属蛋白酶-9（MMP-9）在肝细胞肝癌组织中的表达与肝细胞肝癌血管生成的关系。方法：采用免疫组织化学法检测63例肝细胞肝癌组织中CD147和MMP-9的表达,光镜下计数（分化抗原105 CD105）标记的微血管密度。结果：63例肝细胞肝癌组织中CD147阳性率为65.1%,MMP-9阳性率73.0%,二者阳性率在肿瘤大小、门静脉癌栓、肿瘤分化和TNM分期间差异均有统计学意义（P〈0.05-P〈0.01）,而在患者的性别、年龄及甲胎蛋白的表达水平间差异无统计学意义（P〉0.05）。CD147和MMP-9阳性组微血管密度值均明显高于阴性组（P〈0.01）。CD147在肝细胞肝癌中的表达与MMP-9表达呈正相关关系（P〈0.01）。结论：肝细胞肝癌组织中CD147表达上调可能通过促进MMP-9的生成参与肝癌微血管的生成,进而有利于肝癌侵袭和转移。

Objective：To explore the expressions of CD147 and matrix metalloproteinases-9 in hepatocellular carcinoma, and its correlation with angiogenesis. Methods：The expressions of CD147 and MMP-9 in hepatocellular carcinoma tissues of 63 cases were detected using immunohistochemistry SP method. The microvessel density labeled by differentiation antigen 105（CD105） was recorded under optical microscope. Results：The positive expression rates of CD147 and MMP-9 in hepatocellular carcinoma tissues of 63 cases were 65. 1% and 73. 0%,respectively. The differences of positive expression rates of CD147 and MMP-9 in tumor size,portal vein cancer embolism,tumor differentiation and TNM stage were statistically significant（P〈0. 05 to P 〈0. 01）,but their differences in gender,age and serum level ofα-fetal protein were not statistically significant（P〉0. 05）. The level of microvessel density in case with positive CD147 and MMP-9 expressions was significantly higher than that in case with negative CD147 and MMP-9 expressions （ P〈0.01）. The expressions of CD147 and MMP-9 were significantly positive correlation in hepatocellular carcinoma（P 〈 0. 01）. Conclusions：Upregulation of the CD147 expression in hepatocellular carcinoma can promote angiogenesis through increasing the production of MMP-9,which can lead to tumor invasion and metastasis.