羌活水提取物对肥大细胞活化的影响

Effect of water extract of notopterygium on mast cell activation

目的：探讨羌活水提取物（EN）对肥大细胞活化的影响及其机制.方法：体外获取大鼠腹腔肥大细胞（RPMC）,观察EN不同剂量对anti-DNP IgE诱导肥大细胞活化的影响,酶联法检测anti-DNP IgE介导的肥大细胞组胺释放,酶联免疫吸附测定法检测肥大细胞肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）释放,免疫印迹检测丝氨酸/苏氨酸蛋白激酶（Akt）、核转录因子κB（NF-κB） p65蛋白的表达.结果：体外anti-DNP IgE明显促进RPMC组胺,肿瘤坏死因子-α（TNF-α）和白介素6（IL-6）的释放,诱导Akt磷酸化和NF-κB p65活化,羌活水提取物高（200 μg/L）、中（100 μg/L）浓度明显抑制肥大细胞组胺,TNF-α和IL-6的释放,并抑制Akt磷酸化和NF-κB p65活化.结论：羌活水提取物通过调节Akt,NF-κκB p65信号通路抑制肥大细胞介导的过敏性炎症.

Objective： To explore the effect of water extract from notopterygium （EN） on mast cell activation and its possible mechanism. Methods： Rat peritoneal mast ceils （RPMC） were isolated from SD rats and the effect of EN on mast cell activation was detected by anti-DNP IgE. Histamine release from RPMC was measured by EIA. TNF-a and IL-6 release from RPMC was measured by ELISA. The expression of Akt and NF-KB p65 was measured by Western blotting. Results： IgE- mediated RPMC histamine, TNF-a and IL-6 release were significantly increased. Furthermore, Akt phosphorylation and NF- KB 65 activation were significantly increased. EN （200, 100 μg/L） inhibited anti-DNP IgE-mediated histamine, TNF-a and IL-6 release from RPMC. Akt and NF-KB 65 activation was significantly decreased by EN （200, 100 μg/L） in RPMC. Conclusion： All these results indicated that the water extract of notopterygium can regulate Akt and NF-KB activation to inhibit mast cell-mediated allergy inflarnmation