虾青素抑制氧化应激改善陈旧红细胞保存质量

Astaxanthin improves the quality of stored red blood by inhibiting oxidative stress

目的：观察虾青素能否抑制陈旧悬浮红细胞内的氧化应激,改善红细胞保存质量.方法：采集志愿者血液,制备去白悬浮红细胞,将其随机分为4组,对照组加入DMSO,另外3组悬浮红细胞的保存液内加入抗氧化剂虾青素使其终浓度分别为5、10和20 μmol/L,悬浮红细胞于2℃～6℃内保存.保存至28 d、42 d采用倒置荧光显微镜观察悬浮红细胞内活性氧族的表达状况,采用荧光酶标仪测定红细胞内活性氧族的含量,采用硫代巴比妥酸比色法测定红细胞内丙二醛含量,扫描电镜观察红细胞的超微结构,采用化学比色法测定三磷酸腺苷含量,采用紫外测试法测定2,3-二磷酸甘油酸含量.结果：与各自对照组相比,加虾青素的三组储存28 d和42 d悬浮红细胞内活性氧族和丙二醛含量降低,三磷酸腺苷和2,3-二磷酸甘油酸水平升高,改善了红细胞的形态.结论：虾青素可以通过降低储存悬浮红细胞内的氧化应激水平改善红细胞保存质量.

Objective： To observe whether Astaxanthin can inhibit oxidative stress and improve the quality of older red blood cells. Methods： The blood of volunteers was collected to prepare suspended red blood cells without leukocytes. Then the red blood cells were randomly divided into four groups. As the control group, only the dissolved liquid DMSO was added. The ASTA was added into preservation solution of the other three groups and the final concentration were 5μmol/l., 10 μmol/L and 20 μmol/L respectively. The suspended red blood cells were stored at 2℃-6℃. On Day 28 and 42 of storage, the expression of reactive oxygen species in red blood cells was observed under an inverted fluorescence microscope. The content of reactive oxygen species in red blood cells was detected by fluorescence microplate reader. The content of malondialdehyde was detected with TAB method. The content of adenosine triphosphate was detected with chemical chromatometry. The 2, 3- diphosphoglycerate was detected with UV test method. The morphology of red blood ceils was observed by scanning electrQn microscopy. Results： Compared with the control group, Astaxanthin reduced the content of reactive oxygen species and malondialdehyde in red blood ceils of Astaxanthin treated groups, increased the content of adenosine triphosphate and 2, 3- diphosphoglycerate, and improved the morphology of red blood cells. Conclusion： Astaxanthin can improve the quality of stored red blood cells by inhibiting the oxidative stress reaction.