乳腺原发癌和淋巴结转移癌干细胞Hedgehog信号通路相关基因的比较

Comparative study on Hedgehog signaling pathyway related genes in cancer stem cells of primary breast cancer and matched metastasis lymph nodes

目的：探讨Hedgehog信号通路相关基因在乳腺原发癌干细胞与其相应的淋巴结转移癌干细胞中的表达及意义.方法：收集新鲜的乳腺癌标本,经快速病理诊断为乳腺浸润性导管癌,且伴有腋窝淋巴结的转移,采用免疫磁珠分选法分别提取乳腺原发癌与淋巴结转移癌干细胞,采用Real-time PCR检测Hedgehog信号通路相关基因Shh、Ptch、Smo和Gli-1在乳腺原发癌干细胞与相应转移癌干细胞中的表达.结果：Shh在乳腺原发癌干细胞和相应淋巴结转移癌干细胞中的表达没有统计学意义;Ptch在乳腺原发癌干细胞的表达明显高于转移癌干细胞,而Smo、Gli-1在乳腺原发癌干细胞的表达明显低于转移癌干细胞.结论：与乳腺原发癌干细胞相比,转移癌干细胞Hedgehog信号通路处于更高的活化状态,从而具有更强的侵袭和转移能力,有可能成为新的治疗靶点.

Objective： To investigate the related gene expression of Hedgehog signaling pathway in primary breast cancer stem cells and matched lymph node metastasis cancer stem cells. Methods： Thirty cases of fresh breast invasive ductal carcinoma and matched metastasis lymph nodes were made into single cell suspensions by mechanical separation, and then breast cancer stem cells were separated by immunomagnetic sorting. The related gene expressions of Hedgehog signaling pathway of breast cancer stem cells were detected by real-time PCR. Results： The expression of Shh had no differences in primary breast cancer stem cells and matched lymph node metastasis cancer stem cells. The Ptch expression in primary breast cancer stem cells was higher than that in the matched lymph node metastasis cancer stem cells. The Smo and Gli-1 expressions in primary breast cancer stem cells were lower than those in matched lymph node metastasis cancer stem cells. Conclusion： Compared with the primary breast cancer stem cells, the Hedgehog signaling pathway has a higher activated condition in the cancer stem cells of matched lymph node metastases, which leads to higher capability of invasion and metastasis and may be a new therapeutic target.