慢性牙周炎患者唾液中miR-146a的表达及临床意义

Expression and clinical significance of saliva miR-146a in patients with chronic periodontitis

目的通过检测慢性牙周炎患者唾液中miR-146a的表达,以探讨miR-146a在慢性牙周炎发病中的作用及意义。方法选取2012年1月至2013年8月期间第三军医大学西南医院口腔科收治的48例慢性牙周炎患者(其中男性28例,女性20例,平均年龄42岁)。同时选择20名性别、年龄匹配的健康志愿者作为对照组。采用实时荧光定量PCR技术检测上述人员唾液上清中miR-146a的表达水平。对治疗组患者(32例)进行牙周基础治疗,分别记录治疗前和治疗6周后患者的探诊深度(probing depth,PD)、临床附着丧失(attachment loss,AL)及菌斑指数(plaque index,PLI),并检测治疗后唾液上清液标本中miR-146a的表达。结果慢性牙周炎患者唾液上清液中miR-146a的表达水平(2.52±0.87)显著高于健康对照组(0.81±0.58)(P<0.01)。治疗组患者经过治疗后的牙周临床指数、唾液中miR-146a表达水平均显著降低(P<0.05)。唾液中miR-146a水平与PD(r=0.574,P<0.05)、AL(r=0.629,P<0.01)及PLI(r=0.758,P<0.01)均呈正相关。结论慢性牙周炎患者唾液上清中miR-146a表达水平明显高于正常人,并在牙周基础治疗后显著下降,提示唾液miR-146可作为牙周病防治及疗效评估的潜在靶分子。

Objective To determine the expression of miR-146a in saliva of patients with chronic periodontitis and investigate its clinical value. Methods Forty-eight chronic periodontitis patients admitted in stomatology of Southwest Hospital from January 2012 to August 2013 were investigated （including 28 males and 20 females at a mean age of 42 years） and 20 age- and sex-matched healthy volunteers were enrolled as healthy controls. Real-time PCR was performed to detect the expression of saliva miR-146a in these subjects. Thirty-two patients with chronic periodontitis were treated with basic periodontal therapy for 6 weeks. Their probing depth （PD）, attachment loss （AL） and plaque index （PLI） were recorded before and after treatment. Results The expression of saliva miR-146a were higher in chronic periodontitis patients than in the healthy controls （2.52 ±0.87 vs0. 81 ±0.58, P 〈0.01 ）. After basic periodontal therapy, the MiR-146a expression and all periodontal indexes were reduced （ P 〈 0.05 ）. Positive correlation was found between saliva miR-146a expression with periodontal PD （r = 0. 574, P 〈 0.05）, AL （r = 0. 629, P 〈 0.01 ） and PLI （ r = 0. 758, P 〈 0.01 ）. Conclusion MiR-146a expression is obviously stronger in saliva of patients with chronic periodontitis than in healthy people, and significantly decreased after initial therapy, indicating that miR-146a may be considered as a potential target for prevention and treatment of chronic periodontitis.