纤连蛋白降解片段对心肌细胞腱糖蛋白C表达的影响

Effects of fibronectin fragments on tenascin-C expression in heart cells

目的:探讨纤连蛋白(fibronectin,FN)降解片段对心肌细胞腱糖蛋白C(tenascin-C,TN-C)再表达的影响。方法:通过构建携带FN片段(包括FN-f1、FN-f2、FN-f3、FN-f4、FN-f5、FN-f6和FN-f7)慢病毒载体转染H9C2心肌细胞,采用免疫荧光及Western blotting检测TN-C的表达和分布情况。结果:相比于对照组,FN-f1、FN-f2、FN-f3和FN-f5组,免疫荧光结果显示TN-C表达升高幅度分别为(2.72±0.11)、(2.51±0.06)、(2.14±0.10)、(2.39±0.09),Western blotting则分别升高(0.415±0.009)、(0.290±0.012)、(0.386±0.014)、(0.332±0.016),TN-C表达显著升高,同时FN表达不受影响。而FN-f4、FN-f6和FN-f7对TN-C及FN的表达均没有影响。结论:部分FN片段可能参与TN-C的再表达和心肌重构的过程。

Objective： To determine which fibronectin （ FN） degradation fragments contribute to the induction of tenascin-C （ TN-C） re-expression. Methods：Lentiviral vectors carrying FN fragments,including FN-f1,FN-f2,FN-f3,FN-f4,FN-f5,FN-f6 and FN-f7 were constructed. The protein expressions of TN-C and FN were measured with immunofluorescence assay and Western blotting. Re-sults：Transfection with FN-f1,FN-f2,FN-f3 and FN-f5 significantly induced TN-C expression but did not affect FN expression in H9C2 cells. However,FN-f4,FN-f6 and FN-f7 did not affect the TN-C expression. Conclusion：Some FN fragments may be involved in TN-C re-expression and the development of ventricular remodeling.