ST6GalⅠ调控胃癌细胞SGC7901增殖迁移能力的初步研究

Investigation of ST6GalⅠon the ability of proliferation and migration in SGC7901 gastric cancer cell

目的:探讨唾液酸转移酶(ST6Gal I)对人胃癌细胞(SGC7901)增殖和迁移能力的影响。方法:采用脂质体2000(Lipofectamine 2000),构建ST6Gal I高表达和ST6Gal I低表达细胞株,以空载体(Vector)作为对照组。采用实时荧光定量PCR(real-time PCR,RT-PCR)检测转染后SGC7901细胞中ST6Gal I的m RNA水平;用细胞CCK-8试剂盒及Transwell小室实验检测转染前后细胞的增殖、迁移情况。结果:成功构建稳定转染单克隆细胞株分别为Vector、X61-4和PS7;与Vector组相比,PS7组的m RNA表达水平显著增加(P<0.05),X61-4组则显著降低(P<0.05);X61-4的细胞增殖明显高于PS7细胞(P<0.05);胃癌细胞ST6Gal I的表达水平与细胞迁移能力成正相关,PS7组的迁移率显著高于X61-4组(P<0.01)和Vector组(P<0.01),X61-4组的迁移率低于Vector组(P<0.05)。结论:细胞实验表明,降低ST6Gal I的表达可以有效促进胃癌SGC7901细胞的增殖能力,而上调ST6Gal I可以增强胃癌细胞的迁移能力。因此,ST6Gal I是与肿瘤增殖和转移相关的基因,是潜在的肿瘤分子治疗靶点。

Objective： To explore the effect of α2, 6 sialytransferase （ST6Gal I） on the abilities of proliferation and migration in SGC7901 cells. Methods： Gastric cancer cell lines SGC7901 were con-structed to ST6Gal I high expression （PS7） and ST6Gal I lower expression （X61） with lipofectamine 2000 and empty vector （Vector） was used as a control group. The expression of ST6Gal I was examined with RT-PCR. Cell proliferation was evaluated with the CCK-8 kit, and migration was evaluated with Transwell chamber assay. Results： The 3 stable transfected cell lines were constructed successfully. The most effec-tive clone cells were chosed as our experimental cells, and named as Vector, X61-4 and PS7. In contrasted with Vector, the expression of ST6GAL I in X61-4 was statistically significant lower （P〈0.05）, and PS7 was statistically significant higher （P〈0.05）. Cell growth curve showed that cell proliferation of X61-4 was obviously higher than that of PS7 cells （P〈0.05）. The transwell chamber assay results showed that the migra-tion of PS7 was signiifcantly higher than that of the X61-4 （P〈0.01） and Vector （P〈0.01）, X61-4 was lower than that of the Vector （P〈0.05）. Conclusion：Cell experiment results show that lower ST6Gal I can effectively pro-mote the proliferation ability of SGC7901 gastric cancer cells, but high ST6Gal I can enhance gastric cancer cell migration ability. Therefore, ST6Gal I is related to the tumor proliferation and metastasis, and it is also a potential tumor molecular therapeutic targets.