5-羟色胺及其受体亚型在慢性前列腺炎大鼠脊髓的差异性表达

Differential expression of serotonin and its receptor subtypes in spinal cord of chronic prostatitis model in rat

目的研究5-HT和5-HT2A、5-HT2C、5-HT5A和5-HTR7受体（5-HTR2A、5-HTR2C、5-HTR5A和5-HTR7）在慢性前列腺炎/慢性盆腔疼痛综合征（CP/CPPS）大鼠T13~S1脊髓节段的差异性表达。方法将SD大鼠随机分为对照组、45 d、60 d和90 d模型组,用自身免疫法构建CP/CPPS模型。用免疫组织化学及RT-PCR法检测脊髓T13~L2、L3~L4和L5~S1节段5-HT及各受体的表达。结果 CP/CPPS大鼠自发性疼痛行为学评分显著高于对照组（P〈0.05）。5-HT在各模型组大鼠脊髓3个节段表达均下降。在T13~L2节段,5-HTR2A、5-HTR5A的表达持续上调（P〈0.05）,5-HT2C、5-HTR7先升高后下降（P〈0.05）;在L3~L4节段各受体表达均先升高后下降（P〈0.05）;在L5~S1节段,5-HT2C、5-HTR5A的表达持续上调（P〈0.05）,5-HTR2A、5-HTR7先升高后下降（P〈0.05）。结论 5-HT及5-HTR2A、5-HTR2C、5-HTR5A和5-HTR7在不同时程CP/CPPS大鼠脊髓出现差异性表达,提示5-HT及其受体参与了CP/CPPS的中枢调控,其具体机制有待进一步的研究。

Objective To study the different expressions of serotonin（ 5-HT） and serotonin receptor subtypes（ 5-HTRs） in T13 ~ S1 spinal segments of chronic prostatitis rat models. Methods Sprague Dawley rats were randomly divided into control group,45 d,60 d and 90 d model groups. Chronic prostatitis / chronic pelvic pain syndrome of rats were induced by autoimmune. The level of 5-HT was detected by immunohistochemistry and the expression of5-HTRs gene were detected by RT-PCR in T13 ~ L2,L3 ~ L4,L5 ~ S1 spinal segments in chronic prostatitis rats.Results The spontaneous pain behavior score of CP / CPPS rats was significantly higher as compared with control group（ P〈0. 05）. The levels of 5-HT in three spinal segments in all CP / CPPS model groups were decreased,45 d and 90 d model group decreased significantly（ P〈0. 05）. The expression of 5-HTR2 A,5-HTR5 A showed a gradually increasing and 5-HT2 C,5-HTR7 were increased at the first and then decreased in T13 ~ L2 spinal segments（ P〈0. 05）. The expressions of all receptors were decline after rising first in L3 ~ L4 spinal segments（ P〈0. 05）.The expression of 5-HT2 C,5-HTR5 A showed a gradually increasing and 5-HTR2 A,5-HTR7 were decline after risingfirst in L5 ~ S1 spinal segments（ P〈0. 05）. Conclusions There is a distinct expression pattern of 5-HT and 5-HTRs at different stages of CP / CPPS rat,suggesting that 5-HT amd its receptors may involve in the central regulation mechanism of CP / CPPS,further studies are needed for the specific mechanism.