西藏藏族人群中细小病毒B19基因与内地汉族人群的差异

Different human parvovirus B19 subgroup circulation in Tibet and Han population in China

目的 分析人细小病毒B19及人细小病毒4（PARV4）在西藏藏族人群中与内地汉族人群中的基因特点.方法 采用建立的B19与PARV4 PCR筛查方法,分别从西藏藏族人群中与内地汉族人群血液标本中获得人细小病毒B19及PARV4的部分基因片段,经纯化、测序后,进行基因进化树分析.结果 共获得10个B19 VP1基因片段序列（西藏,2个;四川,3个;浙江,5个）;10个PAV4ORF1基因片段序列（西藏,2个;四川,2个;云南,1个;浙江,5个）;西藏藏族人群中细小病毒B19基因与内地汉族人群同属基因1A亚型,但为不同进化分支;而西藏藏族与内地汉族人群PAV4基因属同一分支.结论 细小病毒B19基因在西藏藏族与内地汉族人群中的差异分析为该病的检测与控制提供了参考.

Objective To investigate the genetic diversity differences of human parvoviruse B19 and parvovirus 4 （PRVA4） in Tibet and Han population in China.Methods Phylogenetic analysis was performed on genome fragments of B19 or PARV4 obtained from the blood samples of Tibet and Han population in China by using a PCR followed by sequencing.Results Ten partial VP1 fragments of B19 （2 from Tibet,3 from Sichuan,5 from Zhejiang） and 10 partial ORF1 fragments of PAV4 （2 from Tibet,2 from Sichuan,1 Yunnan,5 from Zhejiang） were obtained.Phylogenetic analysis indicated that different B19 subgroup circulates in Tibet and Han population although they belong to the same 1A subtype.While the gene evolution of PAV4 is very conserved between the Tibet and Han population in China.Conclusion These studies on genetic diversity of B19 in different Chinese population provide a way for detection and prevention of B19 human parvovirus infection.