ATP结合盒转运蛋白A1在仓鼠和SMB-S15细胞朊病毒感染模型中表达及活性的研究

Expression and activity of ATP-binding cassette transporter A1 （ABCA1） in prion infected hamster and SMB-S15 cell models

目的 评价胆固醇转运子ABCA1在朊病毒病中所发挥的作用.方法 运用WesternBlot检测朊病毒毒株263K感染的仓鼠脑组织和稳定感染羊瘙痒因子Chandler株的小鼠神经细胞系SMB-S15中ABCA1表达的变化,并检测ABCA1激活剂8-Br-cAMP对SMB-S15细胞中朊病毒prpSc含量的影响.结果 与正常对照相比,朊病毒感染的动物及细胞模型中ABCA1蛋白表达显著升高（P＜0.05）,并且在细胞模型中ABCA1活化显著增加prpSc的含量.结论 以上结果提示ABCA1与朊病毒感染的相互关系.该项研究为探索朊病毒复制的分子机制提供了新的思路,同时为以控制胆固醇内稳态为靶点的抗朊病毒治疗提供了理论依据.

Objective To classify the function of the cholesterol transporter ABCA1 in prion infected animal and cell models.Methods The expression of ABCA1 in scrapie strain 263K-infected hamsters and prion infected cell line SMB-S15 was checked by Western Blotting and identify the influence of ABCA1 activation was identified on the level of PrP^Sc in SMB-S15 cells.Results ABCA1 protein expression was down-regulated in scrap ie infection.Moreover,activation of ABCA1 by 8-Br-cAMP increased the amount of PrP^Sc in SMB-S15 cells,demonstrating the role of ABCA1 in prion replication.Conclusions This study further characterized the potential function of ABCA1during prion infection,which will surely bring new insights into prion pathogenesis research and provide new drug target in disease treatment study.