北京地区儿童EBV感染疾病中EBER基因变异特征分析

Characteristics of EBER gene of Epstein-Barr virus in EBV infected children in Beijing area

目的 分析儿童IM、EBV-HLH、CAEBV中EBV EBER基因特征,探讨EBV变异株与EBV感染疾病临床表型间的相关性.方法 应用PCR方法扩增288例IM、52例EBV-HLH和9例CAEBV全血标本的EBNA3C和EBER基因,根据EBNA3C扩增产物的大小进行EBV分型;EBER基因扩增产物经测序后应用BioEdit 7.0.9和Mega4.0.2软件进行分析.统计分析不同EBV亚型和EBER基因型与临床表型的关系.结果 349例标本EBNA3C基因扩增阳性,EBV-Ⅰ/II型的检出率分别为98.6％和1.4％,共检测到EB-6m、EB-8m和EB-7m等3种EBER基因型,其检出率分别为96％、2.9％和1.1％,不同EBER基因型在IM、EBV-HLH、CAEBV中的检出率差异无统计学意义.结论 儿童EBV感染疾病中EBV以EBV-Ⅰ型为主,EB-6m基因型是EBER基因型中的优势基因型.

Objective To analyze the characteristic of EBER gene of Epstein—Barr virus in EBV infection in children in Beijing area.Methods Total DNA was extracted from whole blood of 288 patents with EBV-associated infectious mononucleosis （IM）,52 patients with EBV-associated hemophagocytic lymphohistiocytosis （EBV-HLH） and 9 patients with chronic active Epstein-Barr virus （CAEBV） infection in Beijing Children＇s Hospital.The subtype of EBV type Ⅰ / Ⅱ was defined by the size of amplicon of the EBNA3C genes.The amplified EBER gene products were sequenced directly and the sequences were analyzed by BioEdit 7.0.9 and MEGA 4.0.2.The relationship between EBV subtype or EBER genotype and clinical phenotype were statistically analyzed.Results Type Ⅰ EBV was present in 98.6% samples （344/ 349） and type Ⅱ EBV in 1.4% samples （5/349）.Three EBER genotypes,including EB-6m,EB-8m and EB-7m,were defined in this study.Thc detection rates of EB-6m,EB-8m and EB-7m were 96%,2.9% and 1.1% respectively.There were no significant differences in the frequencies of the three genotypes among IM,EBV-HLH and CAEBV （P 〉 0.05）.Conclusion EBV type Ⅰ is predominant in EBV infection in children in Beijing area.EB-6m genotype was predominant genotype in this area.