摘要
为研究盐生植物小花碱茅(Puccinellia tenuiflora)耐盐的分子机制,本研究根据已克隆到的Pt SKOR基因片段,利用RACE技术得到小花碱茅Pt SKOR的3'和5'c DNA序列,拼接后的基因全长为2 353 bp,编码715个氨基酸,系统进化分析显示Pt SKOR编码外整流K+通道。在此基础上,采用酶切连接的方法,构建了Ca MV 35S启动子驱动的含Pt SKOR基因片段反向重复序列的Pt SKOR-RNAi植物表达载体。
In order to investigate the molecular mechanism of the halophyte plant Puccinellia tenuiflora for salt tolerance, the 3' cDNA sequence and 5' cDNA sequence were cloned with RACE technology based on the reported PtSKOR gene fragment. Sequencing results indicated that the length of the PtSKOR gene from P. tenuiflora was 2 353 bp encoding 715 amino acids. Homology comparison showed that it was the outward-rectifying potassium channel gene named PtSKOR. Then the RNAi plant expression vector PtSKOR-RNAi with inverted repeats of PtSKOR driven by the promoter CaMV 35S was constructed by restriction enzyme digestion and ligation.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第4期877-886,共10页
Molecular Plant Breeding
基金
国家自然科学基金项目(31170431)
教育部博士点基金优先发展领域项目(20130211130001)
兰州大学中央高校基本科研业务费专项资金(lzujbky-2013-239)共同资助