不同肺表面活性物质对兔胸部撞击肺损伤中促炎性细胞因子的影响

Effects of various pulmonary surfactants on proinflammatory cytokines in rabitts with lung injury induced by chest impact

目的观察不同肺表面活性物质(PS)对兔胸部撞击肺损伤后促炎性细胞因子的影响。方法选取新西兰大白兔52只,撞击物以自由落体的方式垂直撞击其右侧胸部建立急性肺损伤模型,随机分为对照组17只、羊PS组17只和牛PS组18只,分别以生理盐水、羊、牛肺表面活性物质行支气管肺泡灌洗,然后处死动物取肺组织;采用酶联免疫吸附(ELISA)法检测肺泡灌洗液和肺组织匀浆中肿瘤坏死因子-α(TNF-α)、白介素-1(IL-1)、白介素-6(IL-6)含量。结果羊PS组及牛PS组的肺泡灌洗液和肺组织匀浆中的TNF-α、IL-1、IL-6含量均低于对照组(P<0.05),而羊PS组与牛PS组的TNF-α、IL-1、IL-6含量比较,差异无统计学意义(P>0.05)。结论羊、牛肺表面活性物质均能有效减少兔急性肺损伤促炎性细胞因子的含量,对急性肺损伤有一定的保护作用。

Objective To observe the effects of various pulmonary surfactant（PS） on the inflammatory cytokines in rabitts with lung injury induced by chest impact. Methods Fifty-two New Zealand white rabbits were selected in the study, and the model of acute lung injury was established by vertical impact on the right chest of the rabbit. The 52 rabbits were randomly divided into control group （ n = 17）, sheep PS group （ n = 17） and cattle PS group （ n = 18 ）, were given bronchoalveolar lavage with normal saline, PS of sheep＇ lung and PS of cattle＇lung, respectively, and then the animals were killed to collect the lung tissues. Enzyme-linked immunosorbent assay （ELISA） was used to detect the contents of tumor nuclear factor（TNF） -α, interleukin（IL） -1 and IL-6 in bronchoalveolar lavage fluid and lung homogenate. Results Sheep PS and cattle PS groups presented significantly lower contents of TNF-α,IL-1 and IL-6 in bronchoalveolar lavage fluid and lung homogenate compared with control group（P 〈 0.05 ）. There was no significant difference in the contents of TNF-α, IL-1 and IL-6 between sheep PS group and cattle PS group（P 〉 0.05）. Conclusion The PS of sheep＇ lung and cattle＇s lung can significantly reduce the content of inflammatory cytokines in rabbits with acute lung injury,which might own certain inhibitory effects on proinflammatory cytokines.