广西汉族人群IL-10-819T/C及IFN-γ+874T/A基因多态性与乙型肝炎病毒感染预后的关系

Relationship of polymorphisms of interleukin-10-819T / C gene and interferon-γ + 874T / A gene with prognosis of hepatitis B virus infection in Guangxi Han nationality

目的探讨白细胞介素10(IL-10)基因819T/C位点及干扰素γ(IFN-γ)基因+874T/A位点单核苷酸多态性与乙型肝炎病毒(HBV)慢性感染不同临床类型的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法及序列特异引物引导的聚合酶链式反应(PCR-SSP)技术检测254例慢性HBV感染者(慢性HBV携带者48例,慢性乙型肝炎患者148例,乙型肝炎肝硬化患者58例)以及健康对照组56例的IL-10基因819 T/C位点和IFN-γ基因+874T/A位点单个核苷酸多态性,观察其在不同临床类型慢性HBV感染者及健康对照者中的分布频率。结果乙型肝炎肝硬化组、慢性乙型肝炎组、携带者组IL-10-819T/C位点基因型分布频率与对照组比较差异无统计意义(P>0.05)。乙型肝炎肝硬化组、慢性乙型肝炎组IL-10-819T/C位点等位基因分布频率与对照组比较差异有统计学意义(P<0.05)。乙型肝炎肝硬化组及慢性乙型肝炎和对照组中IFN-γ基因+874T/A位点基因型及等位基因分布频率比较差异有统计学意义(P<0.05)。携带者组与对照组中IFN-γ基因+874T/A位点基因型及等位基因分布频率差异无统计学意义(P>0.05)。结论 IL-10-819 T等位基因与HBV感染后临床发展过程可能相关;IFN-γ+874T/A基因多态性可能在HBV感染后疾病的发展转归中起重要作用。

Objective To investigate the relationship of the single nucleotide polymorphisms of interleukin-10 gene -819T/C （IL-10-819T/C） and interferon-γ/gene ＋ 874T/A （IFN-T ＋ 847T/A） with different clinical types of chronic hepatitis B virus（HBV） infection. Methods The single nucleotide polymorphisms of IL-10-819T/C and IFN-γ/＋ 847T/A were detected in 254 cases of chronic HBV infection,including 48 chronic HBV carriers, 148 cases of chronic hepatitis B and 58 cases of HBV-related liver cirrhosis, and 56 healthy controls by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and polymerase chain reactionsequence-specific primers（PCR-SSP）. The distribution frequencies of genotype and allele were observed in patients with different clinical types of chronic HBV infection as well as healthy controls. Results The genotype frequencies of IL-10-819T/C of HBV-related liver cirrhosis patients, chronic hepatitis B patients and HBV carrier patients showed no significant difference compared with the controls （P 〉 0.05 ）. The allele frequencies of IL-10-819T/C of HBV-related liver cirrhosis patients and chronic hepatitis B patients showed a significant difference compared with the controls （P 〈 0.05 ）. The frequencies of genotype and allele of IFN-γ/＋ 847T/A of HBV-related liver cirrhosis patients and chronic hepatitis B patients showed a significant difference compared with the controls（P 〈0.05）. There was no significant difference in the frequencies of genotype and allele of IFN-γ ＋ 847T/A between HBV-related liver carrier patients and the controls （ P 〉 0. 05 ）. Conclusion The allele T of IL-10-819 might be associated with the clinical process of HBV infection. The IFN-γ/＋ 874T/A polymorphism may play an important role in the development and outcome of the disease after being infected with HBV.